ArticleJournal of clinical microbiology2005
Sensitivities and specificities of spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat typing methods for studying molecular epidemiology of tuberculosis.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Evaluation of customised lineage-specific sets of MIRU-VNTR loci for genotyping Mycobacterium tuberculosis complex isolates in Ghana.PloS one · 2014Trial
- A Comprehensive Evaluation of GeneLEAD VIII DNA Platform Combined to Deeplex Myc-TBFrontiers in cellular and infection microbiology · 2021Article
- Exploring the usefulness of molecular epidemiology of tuberculosis in Africa: a systematic review.International journal of molecular epidemiology and genetics · 2020Review
- The relationship between transmission time and clustering methods in Mycobacterium tuberculosis epidemiology.EBioMedicine · 2018Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Whole-genome sequencing in outbreak analysis.Clinical microbiology reviews · 2015Review
- Neighborhood socioeconomic position and tuberculosis transmission: a retrospective cohort study.BMC infectious diseases · 2014Article
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Molecular typing of Mycobacterium bovis isolates: a review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Review
- First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance.BMC microbiology · 2010Article
- Multiple-locus variable-number tandem-repeat analysis of Salmonella enterica serovar Typhi.Journal of clinical microbiology · 2009Article
- Major Mycobacterium tuberculosis lineages associate with patient country of origin.Journal of clinical microbiology · 2009Article
- Genetic diversity of isoniazid-resistant Mycobacterium tuberculosis isolates collected in Poland and assessed by spoligotyping.Journal of clinical microbiology · 2008Article
- Multidrug-resistant tuberculosis outbreak among US-bound Hmong refugees, Thailand, 2005.Emerging infectious diseases · 2008Article
- Evaluation of the new advanced 15-loci MIRU-VNTR genotyping tool in Mycobacterium tuberculosis molecular epidemiology studies.BMC microbiology · 2008Article
- Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis.Journal of clinical microbiology · 2007Article
- Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis.Journal of clinical microbiology · 2006Article
- The study recurrent tuberculosis and exogenous reinfection, Shanghai, China.Emerging infectious diseases · 2006Article
- Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology.BMC microbiology · 2006Article
- Low positive predictive values and specificities of spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat typing methods for performing population-based molecular epidemiology studies of tuberculosis.Journal of clinical microbiology · 2005Article
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8 authors.
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Abstract
The development of PCR-based genotyping modalities (spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat [MIRU-VNTR] typing) offers promise for real-time molecular epidemiological studies of tuberculosis (TB). However, the utility of these methods depends on their capacity to appropriately classify isolates. To determine the operating parameters of spoligotyping and MIRU-VNTR typing, we have compared results generated by these newer tests to the standard typing method, IS6110 restriction fragment length polymorphism, in analyses restricted to high-copy-number IS6110 isolates. Sensitivities of the newer tests were estimated as the percentages of isolates with identical IS6110 fingerprints that had identical spoligotypes and MIRU-VNTR types. The specificities of these tests were estimated as the percentages of isolates with unique IS6110 fingerprints that had unique spoligotypes and MIRU-VNTR types. The sensitivity of MIRU-VNTR typing was 52% (95% confidence interval [CI], 31 to 72%), and the sensitivity of spoligotyping was 83% (95% CI, 63 to 95%). The specificity of MIRU-VNTR typing was 56% (95% CI, 51 to 62%), and the specificity of spoligotyping was 40% (95% CI, 35 to 46%). The proportion of isolates estimated to be due to recent transmission was 4% by identical IS6110 patterns, 19% by near-identical IS6110 patterns, 33% by MIRU-VNTR typing, and 53% by spoligotyping. The low calculated specificities of spoligotyping and MIRU-VNTR typing led to misclassification of cases, inflated estimates of TB transmission, and low positive predictive values, suggesting that these techniques have unsuitable operating parameters for population-based molecular epidemiology studies.
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