ArticleJournal of clinical microbiology2005
External quality assessment for molecular detection of Bordetella pertussis in European laboratories.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 67 citations in OpenAlex.
- The 2024 resurgence ofFrontiers in public health · 2025Article
- Challenges and clinical relevance of molecular detection of Bordetella pertussis in South Africa.BMC infectious diseases · 2019Article
- Article
- Comparing the Yield of Nasopharyngeal Swabs, Nasal Aspirates, and Induced Sputum for Detection of Bordetella pertussis in Hospitalized Infants.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Status of pertussis in iran.Jundishapur journal of microbiology · 2014Article
- Comparative evaluation of the Diagenode multiplex PCR assay on the BD max system versus a routine in-house assay for detection of Bordetella pertussis.Journal of clinical microbiology · 2014Article
- Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.Iranian journal of microbiology · 2013Article
- Novel multitarget real-time PCR assay for rapid detection of Bordetella species in clinical specimens.Journal of clinical microbiology · 2011Article
- A real-time PCR assay with improved specificity for detection and discrimination of all clinically relevant Bordetella species by the presence and distribution of three Insertion Sequence elements.BMC research notes · 2011Article
- Resemblance and divergence: the "new" members of the genus Bordetella.Medical microbiology and immunology · 2010Review
- Proficiency program for real-time PCR diagnosis of Bordetella pertussis infections in French hospital laboratories and at the French National Reference Center for Whooping Cough and other Bordetelloses.Journal of clinical microbiology · 2009Article
- Article
- Identification and evaluation of new target sequences for specific detection of Bordetella pertussis by real-time PCR.Journal of clinical microbiology · 2008Article
- Multitarget PCR for diagnosis of pertussis and its clinical implications.Journal of clinical microbiology · 2007Article
- Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.Journal of clinical microbiology · 2006Article
- Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection.Journal of clinical microbiology · 2006Article
- In-house nucleic acid amplification assays in research: how much quality control is needed before one can rely upon the results?Journal of clinical microbiology · 2005Review
- Nucleic Acid amplification tests for diagnosis of Bordetella infections.Journal of clinical microbiology · 2005Review
Corrections and comments
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Authors and funding
14 authors at 9 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the PCR for the detection of Bordetella pertussis is routinely performed in diagnostic laboratories, no quality assessment program has so far been described. We report on the results obtained with two external quality assessment proficiency panels sent to European laboratories. The first proficiency panel contained a series of dilutions of three previously characterized B. pertussis clinical isolates and two negative controls. No false-positive results were reported by six laboratories providing seven data sets. The reported limits of detection of the three B. pertussis strains varied between 4 and 4,000, 9 and 9,000, and 3 and 30,000 CFU/ml, respectively. The second proficiency panel, composed of a series of dilutions of reference strains of B. pertussis, B. holmesii, B. hinzii, and B. bronchiseptica, as well as negative controls, was sent to nine laboratories. One laboratory reported a negative result for a sample and reported a B. parapertussis-positive sample to be positive for B. pertussis. By using the B. pertussis-specific target gene pertactin, one laboratory detected B. pertussis with 100% specificity. All other laboratories, which used IS481-based assays, reported positive results for the samples containing B. holmesii and B. bronchiseptica, species that have occasionally been recovered from human respiratory samples. These data show that the choice of the target gene is particularly critical for the species specificity of B. pertussis PCR assays.
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