ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Activity dependence of cortical axon branch formation: a morphological and electrophysiological study using organotypic slice cultures.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 113 citations in OpenAlex.
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- Epigenetic and Transcriptional Regulation of Spontaneous and Sensory Activity Dependent Programs During Neuronal Circuit Development.Frontiers in neural circuits · 2022Review
- Neuronal Activity Patterns Regulate Brain-Derived Neurotrophic Factor Expression in Cortical CellsFrontiers in neuroscience · 2021Article
- 3-D multi-electrode arrays detect early spontaneous electrophysiological activity in 3-D neuronal-astrocytic co-cultures.Biomedical engineering letters · 2020Article
- A Simple and Efficient Method for Visualizing Individual CellsFrontiers in neural circuits · 2020Article
- Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures.Journal of visualized experiments : JoVE · 2018Article
- Nucleocytoplasmic Shuttling of Histone Deacetylase 9 Controls Activity-Dependent Thalamocortical Axon Branching.Scientific reports · 2017Article
- Activity-Dependent Dynamics of the Transcription Factor of cAMP-Response Element Binding Protein in Cortical Neurons Revealed by Single-Molecule Imaging.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Brain white matter structure and COMT gene are linked to second-language learning in adults.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Perfused drop microfluidic device for brain slice culture-based drug discovery.Biomedical microdevices · 2016Article
- Altered iPSC-derived neurons' sodium channel properties in subjects with Monge's disease.Neuroscience · 2015Article
- MicroRNA-29a modulates axon branching by targeting doublecortin in primary neurons.Protein & cell · 2014Article
- Homeostatic structural plasticity increases the efficiency of small-world networks.Frontiers in synaptic neuroscience · 2014Article
- Role of EphA/ephrin--a signaling in the development of topographic maps in mouse corticothalamic projections.The Journal of comparative neurology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of neuronal activity on cortical axon branching was studied by imaging axons of layer 2/3 neurons in organotypic slice cultures of rat visual cortex. Upper layer neurons labeled by electroporation of plasmid encoding yellow fluorescent protein were observed by confocal microscopy. Time-lapse observation of single-labeled axons showed that axons started to branch after 8-10 d in vitro. Over the succeeding 7-10 d, branch complexity gradually increased by both growth and retraction of branches, resulting in axon arbors that morphologically resembled those observed in 2- to 3-week-old animals. Electrophysiological recordings of neuronal activity in the upper layers, made using multielectrode dishes, showed that the frequency of spontaneous firing increased dramatically approximately 10 d in vitro and remained elevated at later stages. To examine the involvement of spontaneous firing and synaptic activity in branch formation, various blockers were applied to the culture medium. Cultures were silenced by TTX or by a combination of APV and DNQX but exhibited a homeostatic recovery of spontaneous activity over several days in the presence of blockers of either NMDA-type or non-NMDA-type glutamate receptors alone. Axonal branching was suppressed by TTX and AMPA receptor blockade but not by NMDA receptor blockade. We conclude that cortical axon branching is highly dynamic and that neural activity regulates the early developmental branching of upper layer cortical neurons through the activation of AMPA-type glutamate receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.