ArticleThe EMBO journal1992
RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 64 citations in OpenAlex.
- Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2023Review
- Regulating Expression of Mistranslating tRNAs by Readthrough RNA Polymerase II Transcription.ACS synthetic biology · 2021Article
- Fine-tuning the expression of target genes using a DDI2 promoter gene switch in budding yeast.Scientific reports · 2019Article
- Article
- Utilization of a Strongly InducibleFrontiers in microbiology · 2018Article
- Genomic mining of prokaryotic repressors for orthogonal logic gates.Nature chemical biology · 2014Article
- A novel TetR-regulating peptide turns off rtTA-mediated activation of gene expression.PloS one · 2014Article
- Low level genome mistranslations deregulate the transcriptome and translatome and generate proteotoxic stress in yeast.BMC biology · 2012Article
- Tumor inhibition by genomically integrated inducible RNAi-cassettes.Nucleic acids research · 2006Article
- Retrograde movement of tRNAs from the cytoplasm to the nucleus in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The TetR family of transcriptional repressors.Microbiology and molecular biology reviews : MMBR · 2005Review
- Conditional inhibition of cancer cell proliferation by tetracycline-responsive, H1 promoter-driven silencing of PLK1.Oncogene · 2005Article
- Tetracycline-regulated suppression of amber codons in mammalian cells.Molecular and cellular biology · 1998Article
- Construction of an in vivo-regulated U6 snRNA transcription unit as a tool to study U6 function.RNA (New York, N.Y.) · 1998Article
- Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors.Plant molecular biology · 1997Article
- Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.Molecular and cellular biology · 1996Article
- Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus.Journal of virology · 1995Article
- BC1 RNA: transcriptional analysis of a neural cell-specific RNA polymerase III transcript.Molecular and cellular biology · 1995Article
- Article
- tRNA genes as transcriptional repressor elements.Molecular and cellular biology · 1994Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated whether the RNA polymerase III-driven transcription of eukaryotic tRNA genes can be regulated by the prokaryotic tetracycline operator-repressor system. The bacterial tet operator (tetO) was inserted at two different positions (-7 and -46) upstream of a tRNA(Glu) (amber) suppressor gene. Both constructs are transcribed in Saccharomyces cerevisiae and yield functional tRNAs as scored by suppression of an amber nonsense mutation in the met8-1 allele. Controlled expression of Tet repressor was achieved by fusing the bacterial tetR gene to the yeast gal1 promoter. This leads to expression of Tet repressor in yeast on galactose--but not on glucose--containing media. Regulation of the su-tRNA gene with the tetO fragment inserted at position -7 has been demonstrated. Under conditions which allow tetR expression, cells exhibit a met- phenotype. This methionine auxotrophy can be conditionally reverted to prototrophy by adding tetracycline. However, a su-tRNA gene with the tetO fragment inserted at position -46 cannot be repressed. Our results demonstrate clearly that the bacterial repressor protein binds to its operator in the yeast genome. Formation of this complex in the vicinity of the pol III transcription initiation site reduces the level of su-tRNA at least 50-fold as concluded from quantitative primer extension analyses. This indicates for the first time that class III gene expression can be regulated by a DNA binding protein with its target site in the 5'-flanking region and that a prokaryotic repressor can confer regulation of a suitably engineered tRNA gene.
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