ArticleCell stress & chaperones2004
Heat shock treatment protects osmotic stress-induced dysfunction of the blood-brain barrier through preservation of tight junction proteins.
Article in Cell stress & chaperones, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- Inhibition of HSP90 Preserves Blood-Brain Barrier Integrity after Cortical Spreading Depression.Pharmaceutics · 2022Article
- Calcium-mediated oxidative stress: a common mechanism in tight junction disruption by different types of cellular stress.The Biochemical journal · 2017Article
- CD8 T cells protect adult naive mice from JEV-induced morbidity via lytic function.PLoS neglected tropical diseases · 2017Article
- α-Klotho expression determines nitric oxide synthesis in response to FGF-23 in human aortic endothelial cells.PloS one · 2017Article
- Proteomic analysis of mouse choroid plexus cell line ECPC-4 treated with lipid A.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2016Article
- Intestinal epithelial barrier function and tight junction proteins with heat and exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Review
- Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats.Journal of visualized experiments : JoVE · 2016Article
- Tilapia (Oreochromis mossambicus) brain cells respond to hyperosmotic challenge by inducing myo-inositol biosynthesis.The Journal of experimental biology · 2013Article
- HSP27 protects the blood-brain barrier against ischemia-induced loss of integrity.CNS & neurological disorders drug targets · 2013Article
- Interpretation of biological and mechanical variations between the Lowry versus Bradford method for protein quantification.North American journal of medical sciences · 2010Article
- Cannabinoids inhibit HIV-1 Gp120-mediated insults in brain microvascular endothelial cells.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- HSP72 attenuates renal tubular cell apoptosis and interstitial fibrosis in obstructive nephropathy.American journal of physiology. Renal physiology · 2008Article
- Neuroinflammatory response of the choroid plexus epithelium in fatal diabetic ketoacidosis.Experimental and molecular pathology · 2007Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The blood-brain barrier (BBB) is a specialized structure in the central nervous system (CNS), which participates in maintenance of a state of cerebrospinal fluid homeostasis. The endothelial cells of the cerebral capillaries and the tight junctions between them form the basis of the BBB. Research has shown that destruction of the BBB is associated with diseases of the CNS. However, there is little research on how the BBB might be protected. In this study, we used a high osmotic solution (1.6 M D-mannitol) to open the BBB of rats and Evans blue dye as a macromolecular marker. The effect of heat shock treatment was evaluated. The results show that increased synthesis of heat shock protein 72 (Hsp72) was induced in the heated group only. BBB permeability was significantly less in the heat shock-treated group after hyperosmotic shock. The major tight junction proteins, occludin and zonula occludens (ZO)-1, were significantly decreased after D-mannitol treatment in the nonheated group, whereas they were preserved in the heated group. The coimmunoprecipitation studies demonstrated that Hsp72 could be detected in the precipitates of brain extract interacting with anti-ZO-1 antibodies as well as those interacting with anti-occludin antibodies in the heated group. We conclude that the integrity of tight junctions could be maintained by previous heat shock treatment, which might be associated with the increased production of Hsp72.
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