ArticleMolecular and cellular biology2005
Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
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- A proteome-wide yeast degron collection for the dynamic study of protein function.The Journal of cell biology · 2025Article
- Quantitative analysis of septin Cdc10 & Cdc3-associated proteome during stress response in the fungal pathogen Cryptococcus neoformans.PloS one · 2024Article
- The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast.PLoS biology · 2022Article
- Leveraging machine learning essentiality predictions and chemogenomic interactions to identify antifungal targets.Nature communications · 2021Article
- The ASK1 gene regulates the sensitivity of Fusarium graminearum to carbendazim, conidiation and sexual production by combining with βCurrent genetics · 2021Article
- Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit inGenetics · 2019Article
- Strategies and Challenges in Identifying Function for Thousands of sORF-Encoded Peptides in Meiosis.Proteomics · 2018Article
- Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation.Nature communications · 2014Article
- Genome-wide haploinsufficiency screen reveals a novel role for γ-TuSC in spindle organization and genome stability.Molecular biology of the cell · 2013Article
- Review
- Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2012Article
- The requirement for the Dam1 complex is dependent upon the number of kinetochore proteins and microtubules.Current biology : CB · 2011Article
- Dosage suppression genetic interaction networks enhance functional wiring diagrams of the cell.Nature biotechnology · 2011Article
- Deregulated Ras signaling compromises DNA damage checkpoint recovery in S. cerevisiae.Cell cycle (Georgetown, Tex.) · 2010Article
- Towards the prediction of essential genes by integration of network topology, cellular localization and biological process information.BMC bioinformatics · 2009Article
- A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene.Cell · 2009Article
- Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.Molecular biology of the cell · 2008Article
- Production and initial characterization of Dad1p, a component of the Dam1-DASH kinetochore complex.PloS one · 2008Article
- Protein arms in the kinetochore-microtubule interface of the yeast DASH complex.Molecular biology of the cell · 2007Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
DASH is a microtubule- and kinetochore-associated complex required for proper chromosome segregation and bipolar attachment of sister chromatids on the mitotic spindle. We have undertaken a genetic and biochemical analysis of the DASH complex and uncovered a strong genetic interaction of DASH with the Ras/protein kinase A (PKA) pathway. Overexpression of PDE2 or deletion of RAS2 rescued the temperature sensitivity of ask1-3 mutants. Ras2 negatively regulates DASH through the PKA pathway. Constitutive PKA activity caused by mutation of the negative regulator BCY1 is toxic to DASH mutants such as ask1 and dam1. In addition, we have discovered two novel subunits of DASH, Hsk2 and Hsk3 (helper of Ask1), which are microproteins of fewer than 75 amino acids, as dosage suppressors of ask1 mutants. These are essential genes that colocalize with DASH components on spindles and kinetochores and are present in the DASH complex. Mutants in hsk3 arrest cells in mitosis with short spindles and broken spindle structures characteristic of other DASH mutants. Hsk3 is critical for the integrity of the DASH complex because in hsk3 mutants the association of Dam1, Duo1, Spc34, and Spc19 with Ask1 is greatly diminished. We propose that Hsk3 acts to incorporate Ask1 into the DASH complex.
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