ArticleMolecular and cellular biology2005
DNA methyltransferase 3b regulates nerve growth factor-induced differentiation of PC12 cells by recruiting histone deacetylase 2.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 105 citations in OpenAlex.
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- Genomic Imprinting and the Regulation of Postnatal Neurogenesis.Brain plasticity (Amsterdam, Netherlands) · 2017Review
- Folate-dependent methylation of septins governs ciliogenesis during neural tube closure.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Constitutional Epi/Genetic Conditions: Genetic, Epigenetic, and Environmental Factors.Journal of pediatric genetics · 2017Review
- The defining DNA methylation signature of Floating-Harbor Syndrome.Scientific reports · 2016Article
- Identification of Histone Deacetylase 2 as a Functional Gene for Skeletal Muscle Development in Chickens.Asian-Australasian journal of animal sciences · 2016Article
- Profiling placental and fetal DNA methylation in human neural tube defects.Epigenetics & chromatin · 2016Article
- Epigenetic regulation of stemness maintenance in the neurogenic niches.World journal of stem cells · 2015Review
- Targeting histone deacetylases: perspectives for epigenetic-based therapy in cardio-cerebrovascular disease.Journal of geriatric cardiology : JGC · 2015Review
- HDAC signaling in neuronal development and axon regeneration.Current opinion in neurobiology · 2014Review
- Unique functional roles for class I and class II histone deacetylases in central nervous system development and function.International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience · 2013Review
- Nerve growth factor-mediated regulation of low density lipoprotein receptor-related protein promoter activation.Cellular and molecular neurobiology · 2013Article
- Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Epigenetic mechanisms in commonly occurring cancers.DNA and cell biology · 2012Review
Corrections and comments
- Retraction · 2022-04-12Falsification/Fabrication of Data · Investigation by Company/Institution · Misconduct - Official Investigation(s) and/or Finding(s) ·
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
To elucidate the role of epigenetic reprogramming in cell- or tissue-specific differentiation, we explored the role of DNA methyltransferases (Dnmts) in the nerve growth factor (NGF)-induced differentiation of PC12 (pheochromocytoma) cells into neuronal cells. The mRNA and protein levels of de novo methyltransferase Dnmt3b increased, whereas those of Dnmt3a and Dnmt1 decreased, during NGF-induced neurite outgrowth. Dnmt3b localized in the nucleus, as well as in the growing neurites. When the expression of Dnmt3b was inhibited by antisense or small interfering RNA, PC12 cells continued to proliferate and failed to generate neurites. Cells depleted of Dnmt3b were unable to exit the cell cycle even after 6 days of NGF treatment. Furthermore, this failure in differentiation correlated with significant attenuation in tyrosine phosphorylation of TrkA (a marker for NGF-induced differentiation) and reduced the expression of neuronal markers, Hu antigen, and MAP2. The methyl-CpG content of the PC12 genome or the methylation status of repetitive elements was not significantly altered after differentiation and was not affected by Dnmt3b depletion. This was consistent with the ability of the catalytic-site mutant of Dnmt3b to induce differentiation in Dnmt3b-depleted cells after NGF treatment. The Dnmt3b-mediated differentiation was attributed to its N-terminal domain, which recruits histone deacetylase 2 (Hdac2), as demonstrated by (i) impeding of differentiation by the Hdac inhibitors, (ii) facilitation of the differentiation process by overexpression of the N-terminal domain of Dnmt3b, (iii) higher Hdac activity associated with Dnmt3b after NGF treatment, and (iv) coimmunoprecipitation and cosedimentation of Dnmt3b specifically with Hdac2 in a glycerol density gradient. These data indicate a novel role of Dnmt3b in neuronal differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.