ArticleThe Biochemical journal2005
Interactions between Cdc42 and the scaffold protein Scd2: requirement of SH3 domains for GTPase binding.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.The Journal of cell biology · 2024Article
- Functional and structural insights into RAS effector proteins.Molecular cell · 2024Review
- The Arp2/3 complex promotes periodic removal of Pak1-mediated negative feedback to facilitate anticorrelated Cdc42 oscillations.bioRxiv : the preprint server for biology · 2023Article
- Cdc42 GTPase-activating proteins (GAPs) regulate generational inheritance of cell polarity and cell shape in fission yeast.Molecular biology of the cell · 2021Article
- Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast.Scientific reports · 2021Article
- Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast.Cells · 2020Article
- Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase.PLoS biology · 2020Article
- A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis in fission yeast.Journal of cell science · 2019Article
- Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.The Journal of cell biology · 2018Article
- Many roads to symmetry breaking: molecular mechanisms and theoretical models of yeast cell polarity.Molecular biology of the cell · 2017Review
- Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.Molecular biology of the cell · 2016Article
- Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth.Science (New York, N.Y.) · 2012Article
- Spatial control of Cdc42 activation determines cell width in fission yeast.Molecular biology of the cell · 2011Article
- Ras signaling in yeast.Genes & cancer · 2011Article
- Autoinhibition of GEF activity in Intersectin 1 is mediated by the short SH3-DH domain linker.Protein science : a publication of the Protein Society · 2010Article
- Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The multi-domain protein Scd2 acts as a scaffold upon which the small GTPase Cdc42 (cell division cycle 42), its nucleotide-exchange factor Scd1 and the p21-activated kinase Shk1 assemble to regulate cell polarity and the mating response in fission yeast. In the present study, we show using isothermal titration calorimetry that Scd2 binds two molecules of active GTP-bound Cdc42 simultaneously, but independently of one another. The two binding sites have significantly different affinities, 21 nM and 3 microM, suggesting that they play distinct roles in the Shk1 signalling network. Each of the Cdc42-binding sites includes one of the SH3 (Src homology 3) domains of Scd2. Our data indicate that complex formation does not occur in a conventional manner via the conserved SH3 domain ligand-binding surface. Neither of the isolated SH3 domains is sufficient to interact with the GTPase, and they both require adjacent regions to either stabilize their conformations or contribute to the formation of the Cdc42-binding surface. Furthermore, we show that there is no evidence for an intramolecular PX-SH3 domain interaction, which could interfere with SH3 domain function. This work suggests that SH3 domains might contribute directly to signalling through small GTPases and thereby adds another aspect to the diverse nature of SH3 domains as protein-protein-interaction modules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.