ArticleJournal of biomolecular NMR2004
Determination of protein rotational correlation time from NMR relaxation data at various solvent viscosities.
Article in Journal of biomolecular NMR, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Slow methyl axes motions in perdeuterated villin headpiece subdomain probed by cross-correlated NMR relaxation measurements.Magnetochemistry (Basel, Switzerland) · 2023Article
- NMR measurement of biomolecular translational and rotational motion for evaluating changes of protein oligomeric state in solution.European biophysics journal : EBJ · 2022Review
- Room temperature crystallization of amorphous polysiloxane using photodimerization.Chemical science · 2020Article
- Concomitant disorder and high-affinity zinc binding in the human zinc- and iron-regulated transport protein 4 intracellular loop.Protein science : a publication of the Protein Society · 2019Article
- Continuous Fluorescence Depletion Anisotropy Measurement of Protein Rotation.Journal of fluorescence · 2018Article
- A probe to monitor performance of ¹⁵N longitudinal relaxation experiments for proteins in solution.Journal of biomolecular NMR · 2014Article
- Structural dynamics of bio-macromolecules by NMR: the slowly relaxing local structure approach.Progress in nuclear magnetic resonance spectroscopy · 2010Review
- Translational and rotational diffusion of a small globular protein under crowded conditions.The journal of physical chemistry. B · 2009Article
- Detection of nanosecond time scale side-chain jumps in a protein dissolved in water/glycerol solvent.Journal of biomolecular NMR · 2009Article
- Model-free analysis for large proteins at high magnetic field strengths.Journal of biomolecular NMR · 2007Article
- Protein dynamics from NMR: the slowly relaxing local structure analysis compared with model-free analysis.The journal of physical chemistry. A · 2006Article
- Protein-Inhibitor Interaction Studies Using NMR.Applications of NMR spectroscopyArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An accurate determination of the overall rotation of a protein plays a crucial role in the investigation of its internal motions by NMR. In the present work, an innovative approach to the determination of the protein rotational correlation time tau(R) from the heteronuclear relaxation data is proposed. The approach is based on a joint fit of relaxation data acquired at several viscosities of a protein solution. The method has been tested on computer simulated relaxation data as compared to the traditional tau(R) determination method from T(1)/T(2) ratio. The approach has been applied to ribonuclease barnase from Bacillus amyloliquefaciens dissolved in an aqueous solution and deuterated glycerol as a viscous component. The resulting rotational correlation time of 5.56 +/- 0.01 ns and other rotational diffusion tensor parameters are in good agreement with those determined from T(1)/T(2) ratio.
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