ArticleJournal of bacteriology2005
The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Unraveling RNA contribution to the molecular origins of bacterial surface-enhanced Raman spectroscopy (SERS) signals.Scientific reports · 2024Article
- Bacterial adenine cross-feeding stems from a purine salvage bottleneck.The ISME journal · 2024Article
- A purine salvage bottleneck leads to bacterial adenine cross-feeding.bioRxiv : the preprint server for biology · 2023Article
- Characterization of Five Purine Riboswitches in Cellular and Cell-Free Expression Systems.Current microbiology · 2022Article
- Article
- The Functional 3D Organization of Unicellular Genomes.Scientific reports · 2019Article
- Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity.Microbiology (Reading, England) · 2016Article
- Analysis of cepA encoding an efflux pump-like protein in Corynebacterium glutamicum.Journal of microbiology (Seoul, Korea) · 2014Article
- Nucleotides adjacent to the ligand-binding pocket are linked to activity tuning in the purine riboswitch.Journal of molecular biology · 2013Article
- Fluorescence monitoring of riboswitch transcription regulation using a dual molecular beacon assay.Nucleic acids research · 2013Article
- Effects of Mg2+ on the free energy landscape for folding a purine riboswitch RNA.Biochemistry · 2011Article
- Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms.PLoS genetics · 2011Article
- Enhancement of extracellular purine nucleoside accumulation by Bacillus strains through genetic modifications of genes involved in nucleoside export.Journal of industrial microbiology & biotechnology · 2011Article
- Folding of a transcriptionally acting preQ1 riboswitch.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function.Biophysical reviews · 2009Review
- Mutational analysis of the purine riboswitch aptamer domain.Biochemistry · 2007Article
- Core requirements of the adenine riboswitch aptamer for ligand binding.RNA (New York, N.Y.) · 2007Article
- Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.Journal of bacteriology · 2005Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Bacillus subtilis, the expression of genes encoding enzymes and other proteins involved in purine de novo synthesis and salvage is affected by purine bases and phosphoribosylpyrophosphate (PRPP). The transcription of the genes belonging to the PurR regulon is negatively regulated by the PurR protein and PRPP. The expression of the genes belonging to the G-box (XptR) regulon, including the pbuE gene, is negatively regulated by a riboswitch-controlled transcription termination mechanism. The G-box regulon effector molecules are hypoxanthine and guanine. pbuE encodes a purine base efflux pump and is now recognized as belonging to a third purine regulon. The expression of the pbuE gene is positively regulated by a riboswitch that recognizes adenine. Here we show that the expression of pbuE'-lacZ transcriptional fusions are induced by adenine to the highest extent in mutants which do not express a functional PbuE pump. In a mutant defective in the metabolism of adenine, the ade apt mutant, we found a high intracellular level of adenine and constitutive high levels of PbuE. A growth test using a purine auxotroph provided further evidence for the role of PbuE in lowering the intracellular concentration of purine bases, including adenine. Purine analogs also affect the expression of pbuE, which might be of importance for the protection against toxic analogs. In a mutant that overexpresses PbuE, the expression of genes belonging to the PurR regulon was increased. Our findings provide further evidence for important functions of the PbuE protein, such as acting as a pump that lowers the purine base pool and affects the expression of the G-box and PurR regulons, including pbuE itself, and as a pump involved in protection against toxic purine base analogs.
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