ArticleJournal of bacteriology2005
Roles of thioredoxin reductase during the aerobic life of Lactococcus lactis.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review.Gut microbes · 2020Pooled it
- Genomic insights into dominant lactic acid bacteria in spoiled plant-based meat analogues.Current research in food science · 2026Article
- Adaptive laboratory evolution under oxidative stress in lactococcus lactis subsp. lactis LM1009: transcriptomic remodeling and functional enhancement.World journal of microbiology & biotechnology · 2025Article
- Modulating the microbiome as an approach to anticancer drug development.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- PGK1 Regulates Oxidative Stress in Gestational Diabetes Mellitus through the Estradiol-Keap1-Nrf2 Pathway.International journal of biological sciences · 2025Article
- Microbial community structure of plant-based meat alternatives.NPJ science of food · 2024Article
- Antimicrobial Coatings for Medical Textiles via Reactive Organo-Selenium Compounds.Molecules (Basel, Switzerland) · 2023Article
- Article
- Longitudinal Metatranscriptomic Analysis of a Meat Spoilage Microbiome Detects Abundant Continued Fermentation and Environmental Stress Responses during Shelf Life and Beyond.Applied and environmental microbiology · 2020Article
- Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Effects of ccpA gene deficiency in Lactobacillus delbrueckii subsp. bulgaricus under aerobic conditions as assessed by proteomic analysis.Microbial cell factories · 2020Article
- NADH peroxidase plays a crucial role in consuming HBioscience of microbiota, food and health · 2020Article
- Systems Biology - A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria.Frontiers in microbiology · 2019Review
- Adaptation to Aerobic Environment ofFrontiers in microbiology · 2018Article
- Characterization of two Lactococcus lactis zinc membrane proteins, Llmg_0524 and Llmg_0526, and role of Llmg_0524 in cell wall integrity.BMC microbiology · 2015Article
- A New Type of YumC-Like Ferredoxin (Flavodoxin) Reductase Is Involved in Ribonucleotide Reduction.mBio · 2015Article
- Early adaptation to oxygen is key to the industrially important traits of Lactococcus lactis ssp. cremoris during milk fermentation.BMC genomics · 2014Article
- Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness.Microbial cell factories · 2014Article
- Oxidative stress at high temperatures in Lactococcus lactis due to an insufficient supply of Riboflavin.Applied and environmental microbiology · 2013Article
- Current status and emerging role of glutathione in food grade lactic acid bacteria.Microbial cell factories · 2012Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thiol-disulfide bond balance is generally maintained in bacteria by thioredoxin reductase-thioredoxin and/or glutathione-glutaredoxin systems. Some gram-positive bacteria, including Lactococcus lactis, do not produce glutathione, and the thioredoxin system is presumed to be essential. We constructed an L. lactis trxB1 mutant. The mutant was obtained under anaerobic conditions in the presence of dithiothreitol (DTT). Unexpectedly, the trxB1 mutant was viable without DTT and under aerated static conditions, thus disproving the essentiality of this system. Aerobic growth of the trxB1 mutant did not require glutathione, also ruling out the need for this redox maintenance system. Proteomic analyses showed that known oxidative stress defense proteins are induced in the trxB1 mutant. Two additional effects of trxB1 were not previously reported in other bacteria: (i) induction of proteins involved in fatty acid or menaquinone biosynthesis, indicating that membrane synthesis is part of the cellular response to a redox imbalance, and (ii) alteration of the isoforms of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GapB). We determined that the two GapB isoforms in L. lactis differed by the oxidation state of catalytic-site cysteine C152. Unexpectedly, a decrease specific to the oxidized, inactive form was observed in the trxB1 mutant, possibly because of proteolysis of oxidized GapB. This study showed that thioredoxin reductase is not essential in L. lactis and that its inactivation triggers induction of several mechanisms acting at the membrane and metabolic levels. The existence of a novel redox function that compensates for trxB1 deficiency is suggested.
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