ArticleJournal of bacteriology2005
Molecular nature of spontaneous modifications in gacS which cause colony phase variation in Pseudomonas sp. strain PCL1171.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Genome-wide fitness profiling reveals flagellar rotation as an energetic liability during anaerobic maintenance inbioRxiv : the preprint server for biology · 2026Article
- Motility and growth Burkholderia two-component: a new regulatory system of Burkholderia seminalis.Applied microbiology and biotechnology · 2026Article
- Maximizing bacterial survival: integrating sense-and-respond and bet-hedging mechanisms.Trends in microbiology · 2025Review
- Compensatory mutations reducing the fitness cost of plasmid carriage occur in plant rhizosphere communities.FEMS microbiology ecology · 2023Article
- Plasmid fitness costs are caused by specific genetic conflicts enabling resolution by compensatory mutation.PLoS biology · 2021Article
- Genetic factors involved in rhizosphere colonization by phytobeneficialComputational and structural biotechnology journal · 2020Review
- Migration promotes plasmid stability under spatially heterogeneous positive selection.Proceedings. Biological sciences · 2018Article
- Article
- Plasmid stability is enhanced by higher-frequency pulses of positive selection.Proceedings. Biological sciences · 2018Article
- Enhanced Fluorescent Siderophore Biosynthesis and Loss of Phenazine-1-Carboxamide in Phenotypic Variant ofFrontiers in microbiology · 2018Article
- High functional diversity stimulates diversification in experimental microbial communities.Science advances · 2016Article
- Psychrotrophic bacteria in milk: How much do we really know?Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Review
- Importance of positioning for microbial evolution.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A gacS deletion in Pseudomonas aeruginosa cystic fibrosis isolate CHA shapes its virulence.PloS one · 2014Article
- A bacterial symbiont is converted from an inedible producer of beneficial molecules into food by a single mutation in the gacA gene.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Roles of the Gac-Rsm pathway in the regulation of phenazine biosynthesis in Pseudomonas chlororaphis 30-84.MicrobiologyOpen · 2013Article
- Characterisation of the MutS and MutL Proteins from the Pseudomonas avellanae Mismatch Repair (MMR) System.The open microbiology journal · 2012Article
- Spontaneous Gac mutants of Pseudomonas biological control strains: cheaters or mutualists?Applied and environmental microbiology · 2011Article
- Phenotypic and genome-wide analysis of an antibiotic-resistant small colony variant (SCV) of Pseudomonas aeruginosa.PloS one · 2011Article
- The Legionella pneumophila LetA/LetS two-component system exhibits rheostat-like behavior.Infection and immunity · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I colonies showed that in all these cases the phase II phenotype is caused by spontaneous mutations in gacA or/and gacS. Mutation of gac reduced both the length of the lag phase and the generation time. Isolation and sequencing of the gacS genes from the phase II bacteria revealed one insertion as well as several random point mutations, deletions, and DNA rearrangements. Most phase II colonies reverted with a high frequency, resulting in wild-type gacA and gacS genes and a phase I phenotype. Some phase II bacteria retained the phase II phenotype but changed genotypically as a result of (re)introduction of mutations in either gacA or gacS. The reversion of gacA or gacS to the wild type was not affected by mutation of recA and recB. We conclude that in Pseudomonas sp. strain PCL1171, mutations in gacA and gacS are the basis for phase variation from phase I to phase II colonies and that, since these mutations are efficiently removed, mutations in gac result in dynamic switches between the "wild-type" population and the subpopulations harboring spontaneous mutations in gacA and or gacS, thereby enabling both populations to be maintained.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.