ArticleJournal of bacteriology2005
Nature of the promoter activated by C.PvuII, an unusual regulatory protein conserved among restriction-modification systems.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 45 citations in OpenAlex.
- An epigenetic switch activates bacterial quorum sensing and horizontal transfer of an integrative and conjugative element.Nucleic acids research · 2022Article
- Understanding key features of bacterial restriction-modification systems through quantitative modeling.BMC systems biology · 2017Article
- Binding site of restriction-modification system controller protein in Mollicutes.BMC microbiology · 2017Article
- Natural C-independent expression of restriction endonuclease in a C protein-associated restriction-modification system.Nucleic acids research · 2016Article
- Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system.Nucleic acids research · 2016Article
- Structural analysis of DNA binding by C.Csp231I, a member of a novel class of R-M controller proteins regulating gene expression.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Type II restriction endonucleases--a historical perspective and more.Nucleic acids research · 2014Review
- Article
- To be or not to be: regulation of restriction-modification systems and other toxin-antitoxin systems.Nucleic acids research · 2014Review
- Posttranscriptional self-regulation by the Lyme disease bacterium's BpuR DNA/RNA-binding protein.Journal of bacteriology · 2013Article
- Naturally-occurring, dually-functional fusions between restriction endonucleases and regulatory proteins.BMC evolutionary biology · 2013Article
- Structural analysis of DNA-protein complexes regulating the restriction-modification system Esp1396I.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- A bistable hysteretic switch in an activator-repressor regulated restriction-modification system.Nucleic acids research · 2013Article
- The structural basis of differential DNA sequence recognition by restriction-modification controller proteins.Nucleic acids research · 2012Article
- Recognition of dual symmetry by the controller protein C.Esp1396I based on the structure of the transcriptional activation complex.Nucleic acids research · 2012Article
- A novel restriction-modification system is responsible for temperature-dependent phage resistance in Listeria monocytogenes ECII.Applied and environmental microbiology · 2012Article
- Recognition of DNA by the helix-turn-helix global regulatory protein Lrp is modulated by the amino terminus.Journal of bacteriology · 2011Article
- Antisense RNA associated with biological regulation of a restriction-modification system.Nucleic acids research · 2011Article
- Structural analysis of a novel class of R-M controller proteins: C.Csp231I fromJournal of molecular biology · 2011Article
- Translational independence between overlapping genes for a restriction endonuclease and its transcriptional regulator.BMC molecular biology · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of the protective methyltransferase when the genes enter a new cell. C proteins bind to conserved sequences called C boxes. In the PvuII system, the C boxes have been reported to extend from -23 to +3 relative to the transcription start for the gene for the C protein, an unexpected starting position relative to a bound activator. This study suggests that transcript initiation within the C boxes represents initial, C-independent transcription of pvuIICR. The major C protein-dependent transcript appears to be a leaderless mRNA starting farther downstream, at the initiation codon for the pvuIIC gene. This conclusion is based on nuclease S1 transcript mapping and the effects of a series of nested deletions in the promoter region. Furthermore, replacing the region upstream of the pvuIIC initiation codon with a library of random oligonucleotides, followed by selection for C-dependent transcription, yielded clones having sequences that resemble -10 promoter hexamers. The -35 hexamer of this promoter would lie within the C boxes. However, the spacing between C boxes/-35 and the apparent -10 hexamer can be varied by +/-4 bp with little effect. This suggests that, like some other activator-dependent promoters, PpvuIICR may not require a -35 hexamer. Features of this transcription activation system suggest explanations for its broad host range.
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