ArticleGenomics, proteomics & bioinformatics2004
Post-transcriptional gene silencing induced by short interfering RNAs in cultured transgenic plant cells.
Article in Genomics, proteomics & bioinformatics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Genetic analysis of EMS-induced AGO5 mutants affecting seed coat pigmentation in soybean (Glycine max L.).BMC plant biology · 2026Article
- Gold-Nanocluster-Mediated Delivery of siRNA to Intact Plant Cells for Efficient Gene Knockdown.Nano letters · 2021Article
- Engineering DNA nanostructures for siRNA delivery in plants.Nature protocols · 2020Article
- TDP-43 prevents retrotransposon activation in the Drosophila motor system through regulation of Dicer-2 activity.BMC biology · 2020Article
- Carbon nanocarriers deliver siRNA to intact plant cells for efficient gene knockdown.Science advances · 2020Article
- DNA nanostructures coordinate gene silencing in mature plants.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- RNA Interference for Functional Genomics and Improvement of Cotton (Gossypium sp.).Frontiers in plant science · 2016Review
- On the origin and functions of RNA-mediated silencing: from protists to man.Current genetics · 2006Review
- Non-destructive analysis of the nuclei of transgenic living cells using laser tweezers and near-infrared raman spectroscopic technique.Genomics, proteomics & bioinformatics · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Short interfering RNA (siRNA) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. Two siRNA fragments (siRNA-a and -b), which were designed against different specific areas of coding region of the same target green fluorescent protein (GFP) gene, were used to silence GFP expression in cultured gfp transgenic cells of rice (Oryza sativa L.; OS), cotton (Gossypium hirsutum L.; GH), Fraser fir [Abies fraseri (Pursh) Poir; AF], and Virginia pine (Pinus virginiana Mill.; PV). Differential gene silencing was observed in the bombarded transgenic cells between two siRNAs, and these results were consistent with the inactivation of GFP confirmed by laser scanning microscopy, Northern blot, and siRNA analysis in tested transgenic cell cultures. These data suggest that siRNA-mediated gene inactivation can be the siRNA specific in different plant species. These results indicate that siRNA is a highly specific tool for targeted gene knockdown and for establishing siRNA-mediated gene silencing, which could be a reliable approach for large-scale screening of gene function and drug target validation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.