ArticleNucleic acids research2004
Expression of the sorghum 10-member kafirin gene cluster in maize endosperm.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Advances in Extraction, Structure, and Physiochemical Properties of Sorghum Kafirin for Biomaterial Applications: A Review.Journal of functional biomaterials · 2024Review
- Genome-wide association analysis reveals seed protein loci as determinants of variations in grain mold resistance in sorghum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences.Plant cell reports · 2015Article
- Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.Genetics · 2013Article
- Article
- Improved nutritive quality and salt resistance in transgenic maize by simultaneously overexpression of a natural lysine-rich protein gene, SBgLR, and an ERF transcription factor gene, TSRF1.International journal of molecular sciences · 2013Article
- Construction of a plant-transformation-competent BIBAC library and genome sequence analysis of polyploid Upland cotton (Gossypium hirsutum L.).BMC genomics · 2013Article
- Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research.Nature protocols · 2012Article
- Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research.Nature protocols · 2012 · on this mapArticle
- Expression pattern of the alpha-kafirin promoter coupled with a signal peptide from Sorghum bicolor L. Moench.Journal of biomedicine & biotechnology · 2012Article
- Allelic variation of the β-, γ- and δ-kafirin genes in diverse Sorghum genotypes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- RNA interference-mediated change in protein body morphology and seed opacity through loss of different zein proteins.Plant physiology · 2010Article
- Organization of the prolamin gene family provides insight into the evolution of the maize genome and gene duplications in grass species.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors.Plant molecular biology · 2008Article
- Engineered plant minichromosomes: a bottom-up success?The Plant cell · 2008Article
- Article
- Transformation of rice with long DNA-segments consisting of random genomic DNA or centromere-specific DNA.Transgenic research · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional analysis of chromosomal segments containing linked genes requires the insertion of contiguous genomic sequences from bacterial artificial chromosomes (BACs) into the genome. Therefore, we introduced a 90-kb large BAC clone carrying a 10-copy tandem array of kafirin storage protein genes from sorghum linkage group J, mixed with a selectable marker gene, directly into maize cells using the particle bombardment method. Transgenic plants were regenerated and seeds from eight different transgenic lines were produced. One such transgenic plant was selected that had the entire kafirin gene cluster on a single continuous DNA fragment spanning more than 45 kb integrated into its genome. When alcohol-soluble proteins from individual T2 and T3 seeds of this event were analyzed, significant levels of kafirin were found in addition to the endogenous zein storage proteins, demonstrating that the large exogenous DNA segment is stably integrated into the maize genome and expressed at high levels in subsequent generations. Therefore, we could provide a new utility of plant transformation by the particle bombardment method for functional genomics of multigene families and the modification of the nutritive quality of cereal grains. Despite a tandem array of highly homologous sequences at the transgenic locus, no gene silencing was observed, probably owing to the effects of co-transformed flanking sequences. The expression studies of the transgenic locus also revealed new features of storage protein gene promoters that differed from previous transient gene expression studies, thereby illustrating the significance of the concentration and configuration of DNA-protein interactions in the regulation of gene expression.
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