ArticleGenes & development2005
Exonic splicing enhancers in fission yeast: functional conservation demonstrates an early evolutionary origin.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- GPATCH11 ortholog Sap34 regulates pre-mRNA splicing by interacting with early spliceosomal complexes in Schizosaccharomyces pombe.Scientific reports · 2026Article
- The rice blast fungus SR protein 1 regulates alternative splicing with unique mechanisms.PLoS pathogens · 2022Article
- Article
- Exploring the suitability of RanBP2-type Zinc Fingers for RNA-binding protein design.Scientific reports · 2019Article
- The power of fission: yeast as a tool for understanding complex splicing.Current genetics · 2017Review
- Article
- Interconnections Between RNA-Processing Pathways Revealed by a Sequencing-Based Genetic Screen for Pre-mRNA Splicing Mutants in Fission Yeast.G3 (Bethesda, Md.) · 2016Article
- Widespread alternative and aberrant splicing revealed by lariat sequencing.Nucleic acids research · 2015Article
- Regulation of splicing factors by alternative splicing and NMD is conserved between kingdoms yet evolutionarily flexible.Molecular biology and evolution · 2015Article
- Cwf16p Associating with the Nineteen Complex Ensures Ordered Exon Joining in Constitutive Pre-mRNA Splicing in Fission Yeast.PloS one · 2015Article
- Lariat sequencing in a unicellular yeast identifies regulated alternative splicing of exons that are evolutionarily conserved with humans.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Evidence for deep phylogenetic conservation of exonic splice-related constraints: splice-related skews at exonic ends in the brown alga Ectocarpus are common and resemble those seen in humans.Genome biology and evolution · 2013Article
- A dominant role for meiosis-specific 3' RNA processing in controlling expression of a fission yeast cyclin gene.RNA (New York, N.Y.) · 2012Article
- A U1-U2 snRNP interaction network during intron definition.Molecular and cellular biology · 2012Article
- Why eukaryotic cells use introns to enhance gene expression: splicing reduces transcription-associated mutagenesis by inhibiting topoisomerase I cutting activity.Biology direct · 2011Article
- Specific splicing defects in S. pombe carrying a degron allele of the Survival of Motor Neuron gene.The EMBO journal · 2010Article
- A premature termination codon mutation at the C terminus of foamy virus Gag downregulates the levels of spliced pol mRNA.Journal of virology · 2008Article
- Cross-kingdom patterns of alternative splicing and splice recognition.Genome biology · 2008Article
- Finding exonic islands in a sea of non-coding sequence: splicing related constraints on protein composition and evolution are common in intron-rich genomes.Genome biology · 2008Article
- Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast.The Biochemical journal · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these elements and mediate their communication with the core splicing machinery are conserved and essential in the fission yeast Schizosaccharomyces pombe. Here, we provide evidence that ESE function is conserved through evolution by demonstrating that three exonic splicing enhancers derived from vertebrates (chicken ASLV, mouse IgM, and human cTNT) promote splicing of two distinct S. pombe pre-messenger RNAs (pre-mRNAs). Second, as in extracts from mammalian cells, ESE function in S. pombe is compromised by mutations and increased distance from the 3'-splice site. Third, three-hybrid analyses indicate that the essential SR (serine/arginine-rich) protein Srp2p, but not the dispensable Srp1p, binds specifically to both native and heterologous purine-rich elements; thus, Srp2p is the likely mediator of ESE function in fission yeast. Finally, we have identified five natural purine-rich elements from S. pombe that promote splicing of our reporter pre-mRNAs. Taken together, these results provide strong evidence that the genesis of ESE-mediated splicing occurred early in eukaryotic evolution.
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