ArticleThe American journal of pathology1992
Mature eosinophils stimulated to develop in human-cord blood mononuclear cell cultures supplemented with recombinant human interleukin-5. II. Vesicular transport of specific granule matrix peroxidase, a mechanism for effecting piecemeal degranulation.
Article in The American journal of pathology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 66 citations in OpenAlex.
- Eosinophils: old cells, new directions.Frontiers in medicine · 2024Review
- Galectin-10 as a Potential Biomarker for Eosinophilic Diseases.Biomolecules · 2022Review
- The Enigma of Eosinophil Degranulation.International journal of molecular sciences · 2021Review
- Galectin-10, the protein that forms Charcot-Leyden crystals, is not stored in granules but resides in the peripheral cytoplasm of human eosinophils.Journal of leukocyte biology · 2020Article
- Expression and subcellular localization of the Qa-SNARE syntaxin17 in human eosinophils.Experimental cell research · 2015Article
- Piecemeal degranulation in human eosinophils: a distinct secretion mechanism underlying inflammatory responses.Histology and histopathology · 2010Review
- Functional extracellular eosinophil granules: novel implications in eosinophil immunobiology.Current opinion in immunology · 2009Review
- Vesicle-mediated secretion of human eosinophil granule-derived major basic protein.Laboratory investigation; a journal of technical methods and pathology · 2009Article
- Human eosinophils constitutively express multiple Th1, Th2, and immunoregulatory cytokines that are secreted rapidly and differentially.Journal of leukocyte biology · 2009Article
- Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins.Journal of leukocyte biology · 2008Review
- Mechanisms of eosinophilia in the pathogenesis of hypereosinophilic disorders.Immunology and allergy clinics of North America · 2007Review
- Intragranular vesiculotubular compartments are involved in piecemeal degranulation by activated human eosinophils.Traffic (Copenhagen, Denmark) · 2005Article
- Mechanisms of eosinophil cytokine release.Memorias do Instituto Oswaldo Cruz · 2005Review
- Piecemeal degranulation in human tumour pheochromocytes.Journal of anatomy · 2005Article
- Ultrastructural morphology, cytochemistry, and morphometry of eosinophil granules in Chédiak-Higashi syndrome.The American journal of pathology · 1993Article
- An ultrastructural analysis of tumor-promoting phorbol diester-induced degranulation of human basophils.The American journal of pathology · 1992Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The mechanism of piecemeal degranulation by human eosinophils was investigated. Mature eosinophils that developed in rhIL-5-containing conditioned media from cultured human cord blood mononuclear cells were prepared for ultrastructural studies using a combined technique to image eosinophil peroxidase by cytochemistry in the same sections on which postembedding immunogold was used to demonstrate Charcot-Leyden crystal protein. Vesicular transport of eosinophil peroxidase from the specific granule matrix compartment to the cell surface was associated with piecemeal degranulation. This process involved budding of eosinophil peroxidase-loaded vesicles and tubules from specific granules. Some eosinophil peroxidase that was released from eosinophils remained bound to the cell surface; some was free among the cultured cells. Macrophages and basophils bound the released eosinophil peroxidase to their plasma membranes, internalized it in endocytotic vesicles, and stored it in their respective phagolysosomes and secretory granules. Charcot-Leyden crystal protein was diffusely present in the nucleus and cytoplasm of IL-5-stimulated mature eosinophils. Extensive amounts were generally present in granule-poor and subplasma membrane areas of the cytoplasm in contrast to eosinophil peroxidase, which was secreted and bound to the external surface of eosinophil plasma membranes. These studies establish vesicular transport as a mechanism for emptying the specific eosinophil granule matrix compartment during IL-5-associated piecemeal degranulation.
Indexed as
Identifiers
1562046PMC1886371W200289249What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.