ArticleJournal of virological methods2005
Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples.
Article in Journal of virological methods, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Diagnostic sensitivity and specificity of metagenomic sequencing and qPCR for detection of viruses associated with bovine respiratory disease estimated using Bayesian latent class models.Frontiers in veterinary science · 2026Article
- Molecular investigation and viral load analysis of bovine respiratory syncytial virus in cattle with bovine respiratory disease complex in Inner Mongolia, China.Frontiers in cellular and infection microbiology · 2026Article
- Validation of the Measurement of Beta-Hydroxybutyrate and Non-Esterified Fatty Acids in Bovine Saliva: A Pilot Report.Life (Basel, Switzerland) · 2025Article
- An on-farm observational study on the prevalence and associated factors of bacteremia in preweaned dairy calves diagnosed with bronchopneumonia by thoracic ultrasonography.BMC veterinary research · 2025Article
- Bovine Model of Respiratory Syncytial Virus Infection.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Comparing Occurrence of Bovine Respiratory Pathogens Detected by High-Throughput Real-Time PCR in Nasal Swabs and Non-Endoscopic Bronchoalveolar Lavage Samples from Dairy and Veal Calves.Pathogens (Basel, Switzerland) · 2024Article
- Molecular Characterisation and Antibody Response to Bovine Respiratory Syncytial Virus in Vaccinated and Infected Cattle in Turkey.Pathogens (Basel, Switzerland) · 2024Article
- Article
- Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens.Frontiers in veterinary science · 2021Article
- Infection of calves withCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2020Article
- The pulmonary virome, bacteriological and histopathological findings in bovine respiratory disease from western Canada.Transboundary and emerging diseases · 2020Article
- Article
- Bovine respiratory syncytial virus ISCOMs-Immunity, protection and safety in young conventional calves.Vaccine · 2011Article
- Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine respiratory syncytial virus (BRSV) causes severe disease in naive cattle of all ages and is a common pathogen in the respiratory disease complex of calves. Simplified methods for rapid BRSV diagnosis would encourage sampling during outbreaks and would consequently lead to an extended understanding of the virus. In this study, a BRSV fluorogenic reverse transcription PCR (fRT-PCR) assay, based on TaqMan principle, was developed and evaluated on a large number of clinical samples, representing various cases of natural and experimental BRSV infections. By using a single-step closed-tube format, the turn-around time was shortened drastically and results were obtained with minimal risk for cross-contamination. According to comparative analyses, the detection limit of the fRT-PCR was on the same level as that of a nested PCR and the sensitivity relatively higher than that of a conventional PCR, antigen ELISA (Ag-ELISA) and virus isolation (VI). Interspersed negative control samples, samples from healthy animals and eight symptomatically or genetically related viruses were all negative, confirming a high specificity of the assay. Taken together, the data indicated that the fRT-PCR assay can be applied to routine virus detection in clinical specimens and provides a rapid and valuable tool in BRSV research.
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