ArticlePlant physiology2005
Site preferences of insertional mutagenesis agents in Arabidopsis.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Article
- Molecular genetic characterization ofbioRxiv : the preprint server for biology · 2025Article
- Development of Ac- and Ds-tagged starter lines for large-scale transposon-mutagenesis in tomato.PloS one · 2025Article
- High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes.BMC genomics · 2022Article
- A collection of enhancer trap insertional mutants for functional genomics in tomato.Plant biotechnology journal · 2017Article
- Review
- Production of a high-efficiency TILLING population through polyploidization.Plant physiology · 2013Article
- Spectrum of T-DNA integrations for insertional mutagenesis of Histoplasma capsulatum.Fungal biology · 2013Article
- How important are transposons for plant evolution?Nature reviews. Genetics · 2013Review
- Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery.BMC genomics · 2012Article
- A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.Genes & development · 2011Article
- Genome rearrangements in maize induced by alternative transposition of reversed ac/ds termini.Genetics · 2011Article
- SNP discovery and genetic mapping of T-DNA insertional mutants in Fragaria vesca L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Many or most genes in Arabidopsis transposed after the origin of the order Brassicales.Genome research · 2008Article
- Genome-wide analysis of T-DNA integration into the chromosomes of Magnaporthe oryzae.Molecular microbiology · 2007Article
- Discovery of chemically induced mutations in rice by TILLING.BMC plant biology · 2007Article
- Transposition-based plant transformation.Plant physiology · 2007Article
- Article
- Gene trap lines define domains of gene regulation in Arabidopsis petals and stamens.The Plant cell · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have performed a comparative analysis of the insertion sites of engineered Arabidopsis (Arabidopsis thaliana) insertional mutagenesis vectors that are based on the maize (Zea mays) transposable elements and Agrobacterium T-DNA. The transposon-based agents show marked preference for high GC content, whereas the T-DNA-based agents show preference for low GC content regions. The transposon-based agents show a bias toward insertions near the translation start codons of genes, while the T-DNAs show a predilection for the putative transcriptional regulatory regions of genes. The transposon-based agents also have higher insertion site densities in exons than do the T-DNA insertions. These observations show that the transposon-based and T-DNA-based mutagenesis techniques could complement one another well, and neither alone is sufficient to achieve the goal of saturation mutagenesis in Arabidopsis. These results also suggest that transposon-based mutagenesis techniques may prove the most effective for obtaining gene disruptions and for generating gene traps, while T-DNA-based agents may be more effective for activation tagging and enhancer trapping. From the patterns of insertion site distributions, we have identified a set of nucleotide sequence motifs that are overrepresented at the transposon insertion sites. These motifs may play a role in the transposon insertion site preferences. These results could help biologists to study the mechanisms of insertions of the insertional mutagenesis agents and to design better strategies for genome-wide insertional mutagenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.