ArticleProceedings of the National Academy of Sciences of the United States of America2005
SNP detection using peptide nucleic acid probes and conjugated polymers: applications in neurodegenerative disease identification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 175 citations in OpenAlex.
- Design of mismatch closure for enhanced specificity in DNA strand displacement reactions.Nucleic acids research · 2025Article
- The Promise of Nanotechnology in Personalized Medicine.Journal of personalized medicine · 2022Review
- Enhancement of Probe Density in DNA Sensing by Tuning the Exponential Growth Regime of Polyelectrolyte Multilayers.Chemistry of materials : a publication of the American Chemical Society · 2020Article
- SNP Discrimination by Tolane-Modified Peptide Nucleic Acids: Application for the Detection of Drug Resistance in Pathogens.Molecules (Basel, Switzerland) · 2020Article
- Antisense peptide nucleic acids as a potential anti-infective agent.Journal of microbiology (Seoul, Korea) · 2019Review
- DNA Nanotweezers and Graphene Transistor Enable Label-Free Genotyping.Advanced materials (Deerfield Beach, Fla.) · 2018Article
- Fluorogenic PNA probes.Beilstein journal of organic chemistry · 2018Review
- Single Nucleotide Polymorphism Genotyping in Single-Molecule Electronic Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Recent advances in peptide nucleic acid for cancer bionanotechnology.Acta pharmacologica Sinica · 2017Review
- Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays.Chemical science · 2015Article
- Full genotyping of a highly polymorphic human gene trait by time-resolved fluorescence resonance energy transfer.PloS one · 2014Article
- Visual detection of single-base mismatches in DNA using hairpin oligonucleotide with double-target DNA binding sequences and gold nanoparticles.Biosensors & bioelectronics · 2012Article
- Typing of a polymorphic human gene conferring susceptibility to insulin-dependent diabetes mellitus by picosecond-resolved FRET on non-purified/non-amplified genomic DNA.DNA research : an international journal for rapid publication of reports on genes and genomes · 2012Article
- Fish-on-a-chip: a sensitive detection microfluidic system for Alzheimer's disease.Journal of biomedical science · 2011Review
- Single-nucleotide polymorphism (SNP) genotyping using cationic conjugated polymers in homogeneous solution.Nature protocols · 2009Article
- Fluorescence resonance energy transfer in polydiacetylene liposomes.The journal of physical chemistry. B · 2008Article
- Studies of photoinduced electron transfer and energy migration in a conjugated polymer system for fluorescence "turn-on" chemosensor applications.The journal of physical chemistry. B · 2006Article
- Magnetically assisted DNA assays: high selectivity using conjugated polymers for amplified fluorescent transduction.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A strategy employing a combination of peptide nucleic acid (PNA) probes, an optically amplifying conjugated polymer (CP), and S1 nuclease enzyme is capable of detecting SNPs in a simple, rapid, and sensitive manner. The recognition is accomplished by sequence-specific hybridization between the uncharged, fluorescein-labeled PNA probe and the DNA sequence of interest. After subsequent treatment with S1 nuclease, the cationic water soluble CP electrostatically associates with the remaining anionic PNA/DNA complex, leading to sensitized emission of the labeled PNA probe via FRET from the CP. The generation of fluorescent signal is controlled by strand-specific electrostatic interactions and is governed by the complementarity of the probe/target pair. To assess the method, we compared the ability of the sensor system to detect normal, wild-type human DNA sequences, and those sequences containing a single base mutation. Specifically, we examined a PNA probe complementary to a region of the gene encoding the microtubule associated protein tau. The probe sequence covers a known point mutation implicated in a dominant neurodegenerative dementia known as frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17), which has clinical and molecular similarities to Alzheimer's disease. By using an appropriate PNA probe, the conjugated polymer poly[(9,9-bis(6'-N,N,N-trimethylammoniumhexylbromide)fluorene)-co-phenylene] and S1 nuclease, unambiguous FRET signaling is achieved for the wild-type DNA and not the mutant sequence harboring the SNP. Distance relationships in the CP/PNA assay are also discussed to highlight constraints and demonstrate improvements within the system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.