ArticleThe Journal of physiology2005
Microelectrode array recordings of cultured hippocampal networks reveal a simple model for transcription and protein synthesis-dependent plasticity.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed, 154 citations in OpenAlex.
- A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation.Cell reports methods · 2023Article
- Assaying Chemical Long-Term Potentiation in Human iPSC-Derived Neuronal Networks.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Anesthetics inhibit phosphorylation of the ribosomal protein S6 in mouse cultured cortical cells and developing brain.Frontiers in aging neuroscience · 2023Article
- Development of a platform to investigate long-term potentiation in human iPSC-derived neuronal networks.Stem cell reports · 2022Article
- The microbiota-gut-brain axis and epilepsy from a multidisciplinary perspective: Clinical evidence and technological solutions for improvement of in vitro preclinical models.Bioengineering & translational medicine · 2022Review
- Dysregulation ofLearning & memory (Cold Spring Harbor, N.Y.) · 2022Article
- A functional network of highly pure enteric neurons in a dish.Frontiers in neuroscience · 2022Article
- Flexibility ofFrontiers in cellular neuroscience · 2022Article
- Comparative study between radiofrequency-induced and muscimol-induced inhibition of cultured networks of cortical neuron.PloS one · 2022Article
- The Future of Neurotoxicology: A Neuroelectrophysiological Viewpoint.Frontiers in toxicology · 2021Article
- Altered network properties in C9ORF72 repeat expansion cortical neurons are due to synaptic dysfunction.Molecular neurodegeneration · 2021Article
- The role of the genome in experience-dependent plasticity: Extending the analogy of the genomic action potential.Proceedings of the National Academy of Sciences of the United States of America · 2020Review
- A microscopy-based small molecule screen in primary neurons reveals neuroprotective properties of the FDA-approved anti-viral drug Elvitegravir.Molecular brain · 2020Article
- Certain ortho-hydroxylated brominated ethers are promiscuous kinase inhibitors that impair neuronal signaling and neurodevelopmental processes.The Journal of biological chemistry · 2020Article
- Activation of nicotinic acetylcholine receptors induces potentiation and synchronization within in vitro hippocampal networks.Journal of neurochemistry · 2020Article
- Activity-Dependent Remodeling of Synaptic Protein Organization Revealed by High Throughput Analysis of STED Nanoscopy Images.Frontiers in neural circuits · 2020Article
- Neurogranin Expression Is Regulated by Synaptic Activity and Promotes Synaptogenesis in Cultured Hippocampal Neurons.Molecular neurobiology · 2019Article
- Unique Features of Network Bursts Emerge From the Complex Interplay of Excitatory and Inhibitory Receptors in Rat Neocortical Networks.Frontiers in cellular neuroscience · 2019Article
- Pathway sensor-based functional genomics screening identifies modulators of neuronal activity.Scientific reports · 2018Article
- Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simplified cell culture system was developed to study neuronal plasticity. As changes in synaptic strength may alter network activity patterns, we grew hippocampal neurones on a microelectrode array (MEA) and monitored their collective behaviour with 60 electrodes simultaneously. We found that exposure of the network for 15 min to the GABA(A) receptor antagonist bicuculline induced an increase in synaptic efficacy at excitatory synapses that was associated with an increase in the frequency of miniature AMPA receptor-mediated EPSCs and a change in network activity from uncoordinated firing of neurones (lacking any recognizable pattern) to a highly organized, periodic and synchronous burst pattern. Induction of recurrent synchronous bursting was dependent on NMDA receptor activation and required extracellular signal-regulated kinase (ERK)1/2 signalling and translation of pre-existing mRNAs. Once induced, the burst pattern persisted for several days; its maintenance phase (> 4 h) was dependent on gene transcription taking place in a critical period of 120 min following induction. Thus, cultured hippocampal neurones display a simple, transcription and protein synthesis-dependent form of plasticity. The non-invasive nature of MEA recordings provides a significant advantage over traditional assays for synaptic connectivity (i.e. long-term potentiation in brain slices) and facilitates the search for activity-regulated genes critical for late-phase plasticity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.