ArticleJournal of fluorescence2004
Live imaging of glucose homeostasis in nuclei of COS-7 cells.
Article in Journal of fluorescence, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 76 citations in OpenAlex.
- PFKFB3 Connects Glycolytic Metabolism with Endothelial Dysfunction in Human and Rodent Obesity.Antioxidants (Basel, Switzerland) · 2025Article
- Development and optimization of heavy metal lead biosensors in biomedical and environmental applications.Journal of the Chinese Medical Association : JCMA · 2021Review
- FRET-Based Genetically Encoded Nanosensor for Real-Time Monitoring of the Flux of α-Tocopherol in Living Cells.ACS omega · 2021Article
- One-pot HTST synthesis of responsive fluorescent ZnO@apo-enzyme composite microgels for intracellular glucometry.RSC advances · 2020Article
- Quantitative in vivo imaging of neuronal glucose concentrations with a genetically encoded fluorescence lifetime sensor.Journal of neuroscience research · 2019Article
- Consequences of Heterogeneous Crowding on an Enzymatic Reaction: A Residence Time Monte Carlo Approach.ACS omega · 2019Article
- Conversion of Cytochrome P450 2D6 of Human Into a FRET-Based Tool for Real-Time Monitoring of Ajmalicine in Living Cells.Frontiers in bioengineering and biotechnology · 2019Article
- Exploring cells with targeted biosensors.The Journal of general physiology · 2017Review
- Insulin induces an increase in cytosolic glucose levels in 3T3-L1 cells with inhibited glycogen synthase activation.International journal of molecular sciences · 2014Article
- Single-cell imaging tools for brain energy metabolism: a review.Neurophotonics · 2014Article
- High glucose potentiates L-FABP mediated fibrate induction of PPARα in mouse hepatocytes.Biochimica et biophysica acta · 2013Article
- Impact of L-FABP and glucose on polyunsaturated fatty acid induction of PPARα-regulated β-oxidative enzymes.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Generation of circularly permuted fluorescent-protein-based indicators for in vitro and in vivo detection of citrate.PloS one · 2013Article
- Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.PPAR research · 2013Article
- A genetically encoded metabolite sensor for malonyl-CoA.Chemistry & biology · 2012Article
- New technologies for 21st century plant science.The Plant cell · 2012Article
- Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast.Nature protocols · 2011Article
- Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells.Nature protocols · 2011Article
- Construction of a panel of glucose indicator proteins for continuous glucose monitoring.Biosensors & bioelectronics · 2011Article
- Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring subcellular glucose levels deep in tissues can provide new insights into compartmentalization and specialization of glucose metabolism among different cells. As shown previously, a FRET-based glucose-sensor consisting of two GFP-variants and the Escherichia coli periplasmic glucose/galactose binding protein was successfully expressed in the cytosol of COS7-cells and used to determine cytosolic glucose levels. Recording cytosolic fluorescence intensities in cells located in deeper layers of tissues is often difficult due to loss of signal intensity caused by effects of other cell layers on excitation and emission light. These interfering effects may be reduced by restricting fluorophores to occupy only a fraction of the assayed tissue volume. This can be accomplished by confining fluorophores to a sub-compartment of each cell in the tissue, such as the nucleus. The glucose-sensor was targeted to nuclei of COS7-cells. To determine, whether nuclear glucose levels can be used to track cytosolic changes, nuclear glucose concentrations were quantified as the cells were challenged with external glucose over a range of 0.5 to 10 mM and compared to cytosolic levels. Internal glucose concentrations in both compartments were similar, corresponding to approximately 50% of the external concentration. Taken together, these results indicate that nuclear glucose levels can be used to determine cytosolic levels indirectly, permitting more reliable quantification of fluorescence intensities and providing a tool for measurements not only in cell cultures but also in tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.