ArticleGene therapy2005
Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment.
Article in Gene therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 36 citations in OpenAlex.
- Evaluating the effect of food components on the digestion of dietary nucleic acids in human gastric juice in vitro.Food science & nutrition · 2023Article
- DNA Origami Disguises Herpes Simplex Virus 1 Particles and Controls Their Virulence.Molecules (Basel, Switzerland) · 2022Article
- Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells.Scientific reports · 2021Article
- An evolving function of DNA-containing exosomes in chemotherapy-induced immune response.Cell research · 2017Article
- DNA-fragments are transcytosed across CaCo-2 cells by adsorptive endocytosis and vesicular mediated transport.PloS one · 2013Article
- A mechanism of gene amplification driven by small DNA fragments.PLoS genetics · 2012Article
- Article
- Stem cells: promises and realities in cancer research.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2006Article
- Phosphorothioate-stimulated uptake of short interfering RNA by human cells.EMBO reports · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA can be internalized by mammalian cells without taking advantage of helper reagents. Here, we ask whether the spontaneous cellular uptake of double-stranded DNA (dsDNA) occurs in a biologically significant and sequence-dependent way. We describe a combinatorial approach to search for dsDNA sequence segments that are preferentially internalized. A selected dsDNA species was identified and covalently linked to a luciferase expression cassette. The increased apparent cellular uptake of long-chain recombinant DNA accompanied by an increased apparent expression of luciferase provides strong evidence for the view that (i) naked long-chain dsDNA can be taken up spontaneously by mammalian cells, (ii) specific sequences substantially increase this process, and (iii) dsDNA is transported into the nucleus of cells in a bioactive form. Experimental evidence indicates a tissue- or cell-type specificity for this process. This work indicates that, in principle, specific nucleotide sequences can facilitate the introduction of naked dsDNA into target cells of interest, thereby improving existing vector systems and providing a new methodology to study DNA uptake by mammalian cells. The cellular uptake of biologically active genetic material in vivo occurs to be conceivable.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.