ArticleGene therapy2005
Hepatocyte-targeted gene transfer by combination of vascularly delivered plasmid DNA and in vivo electroporation.
Article in Gene therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Advances in gene transfer technologies: comparing viral and non-viral vectors for therapeutic applications.3 Biotech · 2026Review
- CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver.Nature protocols · 2022Review
- Understanding In Vivo Fate of Nucleic Acid and Gene Medicines for the Rational Design of Drugs.Pharmaceutics · 2021Review
- In Vivo Electroporation-Mediated, Intrahepatic Alpha1 Antitrypsin Gene Transfer Reduces Pulmonary Emphysema in Pallid Mice.Pharmaceutics · 2020Article
- Nonviral vectors: we have come a long way.Advances in genetics · 2014Review
- Efficacy and safety of Sleeping Beauty transposon-mediated gene transfer in preclinical animal studies.Current gene therapy · 2011Review
- Discovery of metabolically stabilized electronegative polyacridine-PEG peptide DNA open polyplexes.Bioconjugate chemistry · 2010Article
- Nonviral gene delivery: principle, limitations, and recent progress.The AAPS journal · 2009Review
- Adenovirus serotype 35 vector-mediated transduction following direct administration into organs of nonhuman primates.Gene therapy · 2009Article
- Physical approaches for nucleic acid delivery to liver.The AAPS journal · 2008Review
- Pharmacokinetic considerations regarding non-viral cancer gene therapy.Cancer science · 2008Review
- Nonviral gene delivery: what we know and what is next.The AAPS journal · 2007Review
- Adenovirus serotype 35 vector-mediated transduction into human CD46-transgenic mice.Gene therapy · 2006Article
- Tissue-specific characteristics of in vivo electric gene: transfer by tissue and intravenous injection of plasmid DNA.Pharmaceutical research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To increase transgene expression in the liver, electric pulses were applied to the left lateral lobe after intravenous injection of naked plasmid DNA (pDNA) or pDNA/liver targeting vector complex prepared with galactosylated poly(L-lysine) or galactosylated polyethyleneimine. Electroporation (250 V/cm, 5 ms/pulse, 12 pulses, 4 Hz) after naked pDNA injection dramatically increased the expression up to 200,000-fold; the expression level obtained was significantly greater than that achieved by the combination of pDNA/vector complex and electroporation. We clearly demonstrated that the expression was dependent on the plasma concentration of pDNA at the time when the electric pulses were applied. Separation of liver cells revealed that the distribution of naked pDNA as well as transgene expression was largely selective to hepatocytes in the electroporated lobe. The number of cells expressing transgene product using vascularly administered naked pDNA followed by electroporation was significantly (P<0.01) greater and more widespread than that obtained by local injection of naked pDNA. These results indicate that the application of in vivo electroporation to vascularly administered naked pDNA is a useful gene transfer approach to a large number of hepatocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.