ArticleThe EMBO journal2005
Structural insights into the EB1-APC interaction.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 148 citations in OpenAlex.
- Mechanism of APC truncation involved in colorectal cancer tumorigenesis (Review).Oncology letters · 2025Review
- Liquid-liquid phase separation of microtubule-binding proteins in the regulation of spindle assembly.Cell proliferation · 2024Review
- End-binding protein 1 promotes specific motor-cargo association in the cell body prior to axonal delivery of dense core vesicles.Current biology : CB · 2023Article
- Nuclear elongation during spermiogenesis depends on physical linkage of nuclear pore complexes to bundled microtubules by Drosophila Mst27D.PLoS genetics · 2023Article
- End Binding protein 1 promotes specific motor-cargo association in the cell body prior to axonal delivery of Dense Core Vesicles.bioRxiv : the preprint server for biology · 2023Article
- Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends.Nature cell biology · 2023Article
- MAPping tubulin mutations.Frontiers in cell and developmental biology · 2023Review
- Multivalency ensures persistence of a +TIP body at specialized microtubule ends.Nature cell biology · 2023Article
- Phospho-regulated Bim1/EB1 interactions trigger Dam1c ring assembly at the budding yeast outer kinetochore.The EMBO journal · 2021Article
- SxIP binding disrupts the constitutive homodimer interface of EB1 and stabilizes EB1 monomer.Biophysical journal · 2021Article
- Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth.Molecular biology of the cell · 2020Article
- NMR resonance assignment and structure prediction of the C-terminal domain of the microtubule end-binding protein 3.PloS one · 2020Article
- Mapping multivalency in the CLIP-170-EB1 microtubule plus-end complex.The Journal of biological chemistry · 2019Article
- Article
- Spatial positioning of EB family proteins at microtubule tips involves distinct nucleotide-dependent binding properties.Journal of cell science · 2018Article
- GTP-binding facilitates EB1 recruitment onto microtubules by relieving its auto-inhibition.Scientific reports · 2018Article
- Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker.The Journal of cell biology · 2018Article
- Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites.Scientific reports · 2017Article
- Microtubule plus-end tracking of end-binding protein 1 (EB1) is regulated by CDK5 regulatory subunit-associated protein 2.The Journal of biological chemistry · 2017Article
- An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.Journal of cell science · 2016Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
EB1 proteins bind to microtubule ends where they act in concert with other components, including the adenomatous polyposis coli (APC) tumor suppressor, to regulate the microtubule filament system. We find that EB1 is a stable dimer with a parallel coiled coil and show that dimerization is essential for the formation of its C-terminal domain (EB1-C). The crystal structure of EB1-C reveals a highly conserved surface patch with a deep hydrophobic cavity at its center. EB1-C binds two copies of an APC-derived C-terminal peptide (C-APCp1) with equal 5 microM affinity. The conserved APC Ile2805-Pro2806 sequence motif serves as an anchor for the interaction of C-APCp1 with the hydrophobic cavity of EB1-C. Phosphorylation of the conserved Cdc2 site Ser2789-Lys2792 in C-APCp1 reduces binding four-fold, indicating that the interaction APC-EB1 is post-translationally regulated in cells. Our findings provide a basis for understanding the dynamic crosstalk of EB1 proteins with their molecular targets in eukaryotic organisms.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.