ArticleNucleic acids research2004
Tfg3, a subunit of the general transcription factor TFIIF in Schizosaccharomyces pombe, functions under stress conditions.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Pooled it
- Elongation factor TFIIS is essential for heat stress adaptation in plants.Nucleic acids research · 2022Article
- Taf14 is required for the stabilization of transcription pre-initiation complex in Saccharomyces cerevisiae.Epigenetics & chromatin · 2020Article
- Schizosaccharomyces pombe Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex.Nucleic acids research · 2018Article
- Super elongation complex contains a TFIIF-related subcomplex.Transcription · 2016Article
- Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM.The EMBO journal · 2012Article
- Evidence that RNA polymerase II and not TFIIB is responsible for the difference in transcription initiation patterns between Saccharomyces cerevisiae and Schizosaccharomyces pombe.Nucleic acids research · 2012Article
- The YEATS domain of Taf14 in Saccharomyces cerevisiae has a negative impact on cell growth.Molecular genetics and genomics : MGG · 2010Article
- Fission yeast Iec1-ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism.Molecular and cellular biology · 2010Article
- Rapid annotation of anonymous sequences from genome projects using semantic similarities and a weighting scheme in gene ontology.PloS one · 2009Article
- Basal transcription machinery: role in regulation of stress response in eukaryotes.Journal of biosciences · 2007Review
- Genetic interactions between TFIIF and TFIIS.Genetics · 2006Article
- Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TFIIF is a general transcription factor (GTF) that binds to RNA polymerase II (pol II) for subsequent recruitment of pol II to a promoter. TFIIF of Saccharomyces cerevisiae contains a small subunit, designated Tfg3, in addition to two conserved subunits, TFIIFalpha (Tfg1) and TFIIFbeta (Tfg2). In this study, we characterized Tfg3 of Schizosaccharomyces pombe. Using Tfg3 fused to green fluorescent protein (GFP), we found that Tfg3 is located in nuclei, and it is assembled into the C-terminal domain phosphatase (Fcp1)/TFIIF/pol II complex via interactions with TFIIFalpha and TFIIFbeta. As in the case of S.cerevisiae, Tfg3 in S.pombe forms part of another GTF, namely TFIID. The TFIID complex isolated from S.pombe that had been cultured at elevated temperatures included increased levels of Tfg3. The interaction of recombinant Tfg3 with TATA-binding protein (TBP), the central subunit of TFIID, was temperature-dependent. Moreover, a mutant of S.pombe that lacked the gene for Tfg3 was sensitive to a battery of stresses including temperature up-shift. Starting from a mutant with tfg3- mutation, we isolated five species of multicopy suppressors. Expression levels of the suppressor genes were lower in the mutant cell than in wild-type cell at an elevated temperature. Taken together, we propose that Tfg3 is involved in transcriptional regulation under stress conditions, in particular, at high temperatures.
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