ArticleBiophysical journal2005
lambda-Repressor oligomerization kinetics at high concentrations using fluorescence correlation spectroscopy in zero-mode waveguides.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- High-Throughput Fabrication of Zero-Mode Waveguide Nanoaperture Arrays with Sol-Gel Nanoimprint Lithography for Enhanced Single Molecule Fluorescence Detection.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores.eLife · 2024Article
- Article
- Gold Ion Beam Milled Gold Zero-Mode Waveguides.Nanomaterials (Basel, Switzerland) · 2022Article
- Fast interaction dynamics of G-quadruplex and RGG-rich peptides unveiled in zero-mode waveguides.Nucleic acids research · 2021Article
- Enhancing Single-Molecule Fluorescence Spectroscopy with Simple and Robust Hybrid Nanoapertures.ACS photonics · 2021Article
- Zero-mode waveguides can be made better: fluorescence enhancement with rectangular aluminum nanoapertures from the visible to the deep ultraviolet.Nanoscale advances · 2020Article
- Mixed metal zero-mode guides (ZMWs) for tunable fluorescence enhancement.Nanoscale advances · 2020Article
- Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.Scientific reports · 2020Article
- Nanoaperture fabrication via colloidal lithography for single molecule fluorescence analysis.PloS one · 2019Article
- Zero-Mode Waveguide Nanophotonic Structures for Single Molecule Characterization.Journal of physics D: Applied physics · 2018Article
- Optics-Integrated Microfluidic Platforms for Biomolecular Analyses.Biophysical journal · 2016Review
- Nanoscale volume confinement and fluorescence enhancement with double nanohole aperture.Scientific reports · 2015Article
- Making connections--strategies for single molecule fluorescence biophysics.Current opinion in chemical biology · 2013Review
- Landscape of next-generation sequencing technologies.Analytical chemistry · 2011Review
- Vertical nanopillars for highly localized fluorescence imaging.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Photonic methods to enhance fluorescence correlation spectroscopy and single molecule fluorescence detection.International journal of molecular sciences · 2010Review
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Quantitative transcription factor binding kinetics at the single-molecule level.Biophysical journal · 2009Article
- Fluorescence fluctuations analysis in nanoapertures: physical concepts and biological applications.Histochemistry and cell biology · 2008Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fluorescence correlation spectroscopy (FCS) has demonstrated its utility for measuring transport properties and kinetics at low fluorophore concentrations. In this article, we demonstrate that simple optical nanostructures, known as zero-mode waveguides, can be used to significantly reduce the FCS observation volume. This, in turn, allows FCS to be applied to solutions with significantly higher fluorophore concentrations. We derive an empirical FCS model accounting for one-dimensional diffusion in a finite tube with a simple exponential observation profile. This technique is used to measure the oligomerization of the bacteriophage lambda repressor protein at micromolar concentrations. The results agree with previous studies utilizing conventional techniques. Additionally, we demonstrate that the zero-mode waveguides can be used to assay biological activity by measuring changes in diffusion constant as a result of ligand binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.