ArticleBiophysical journal2005
Detection of non-Brownian diffusion in the cell membrane in single molecule tracking.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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108 citing papers in PubMed, 315 citations in OpenAlex.
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- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
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- Quantitative evaluation of methods to analyze motion changes in single-particle experiments.Nature communications · 2025Article
- Concurrent diffusion of nicotinic acetylcholine receptors and fluorescent cholesterol disclosed by two-colour sub-millisecond MINFLUX-based single-molecule tracking.Nature communications · 2025Article
- Fast Single-Particle Tracking of Membrane Proteins Combined with Super-Resolution Imaging of Actin Nanodomains.Scientific data · 2025Article
- Trajectory Analysis in Single-Particle Tracking: From Mean Squared Displacement to Machine Learning Approaches.International journal of molecular sciences · 2024Review
- A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane.Scientific reports · 2024Article
- Freestanding bilayer microscope for single-molecule imaging of membrane proteins.Science advances · 2024Article
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- Impact of temporal resolution in single particle tracking analysis.Discover nano · 2024Article
- Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology.The Journal of cell biology · 2023Article
- Implications of different membrane compartmentalization models in particle-basedRoyal Society open science · 2023Article
- Recently developed glycosphingolipid probes and their dynamic behavior in cell plasma membranes as revealed by single-molecule imaging.Glycoconjugate journal · 2023Review
- Effect of Protein-Protein Interactions on Translational Diffusion of Spheroidal Proteins.International journal of molecular sciences · 2022Review
- Modeling Receptor Motility along Advecting Lipid Membranes.Membranes · 2022Review
- Heterogeneity of cell membrane structure studied by single molecule tracking.Faraday discussions · 2021Article
- Reconstructing the Intrinsic Statistical Properties of Intermittent Locomotion Through Corrections for Boundary Effects.Bulletin of mathematical biology · 2021Article
48 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecules undergo non-Brownian diffusion in the plasma membrane, but the mechanism behind this anomalous diffusion is controversial. To characterize the anomalous diffusion in the complex system of the plasma membrane and to understand its underlying mechanism, single-molecule/particle methods that allow researchers to avoid ensemble averaging have turned out to be highly effective. However, the intrinsic problems of time-averaging (resolution) and the frequency of the observations have not been explored. These would not matter for the observations of simple Brownian particles, but they do strongly affect the observation of molecules undergoing anomalous diffusion. We examined these effects on the apparent motion of molecules undergoing simple, totally confined, or hop diffusion, using Monte Carlo simulations of particles undergoing short-term confined diffusion within a compartment and long-term hop diffusion between these compartments, explicitly including the effects of time-averaging during a single frame of the camera (exposure time) and the frequency of observations (frame rate). The intricate relationships of these time-related experimental parameters with the intrinsic diffusion parameters have been clarified, which indicated that by systematically varying the frame time and rate, the anomalous diffusion can be clearly detected and characterized. Based on these results, single-particle tracking of transferrin receptor in the plasma membrane of live PtK2 cells were carried out, varying the frame time between 0.025 and 33 ms (0.03-40 kHz), which revealed the hop diffusion of the receptor between 47-nm (average) compartments with an average residency time of 1.7 ms, with the aid of single fluorescent-molecule video imaging.
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