ArticleNucleic acids research2004
Spatially and temporally controlled gene transfer by electroporation into adherent cells on plasmid DNA-loaded electrodes.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 99 citations in OpenAlex.
- In situ electroporation of mammalian cells through SiOScientific reports · 2021Article
- Delivery of Foreign Materials into Adherent Cells by Gold Nanoparticle-Mediated Photoporation.Membranes · 2021Article
- Comparison between direct and reverse electroporation of cells in situ: a simulation study.Physiological reports · 2016Article
- Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.International journal of molecular sciences · 2015Article
- Microfluidic device for stem cell differentiation and localized electroporation of postmitotic neurons.Lab on a chip · 2014Article
- High efficiency, site-specific transfection of adherent cells with siRNA using microelectrode arrays (MEA).Journal of visualized experiments : JoVE · 2012Article
- In situ electroporation of surface-bound siRNAs in microwell arrays.Lab on a chip · 2012Article
- Article
- Site-specific gene transfer with high efficiency onto a carbon nanotube-loaded electrode.Journal of the Royal Society, Interface · 2008Article
- Bioluminescence imaging for assessment and normalization in transfected cell arrays.Biotechnology and bioengineering · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional characterization of human genes is one of the most challenging tasks in current genomics. Owing to a large number of newly discovered genes, high-throughput methodologies are greatly needed to express in parallel each gene in living cells. To develop a method that allows efficient transfection of plasmids into adherent cells in spatial- and temporal-specific manners, we studied electric pulse-triggered gene transfer using a plasmid-loaded electrode. A plasmid was loaded on a gold electrode surface having an adsorbed layer of poly(ethyleneimine), and cells were then plated directly onto this modified surface. The plasmid was detached from the electrode by applying a short electric pulse and introduced into the cells cultured on the electrode, resulting in efficient gene expression, even in primary cultured cells. The location of transfected cells could be restricted within a small area on a micropatterned electrode, showing the versatility of the method for spatially controlled transfection. Plasmid transfection could also be performed in a temporally controlled manner without a marked loss of the efficiency when an electric pulse was applied within 3 days after cell plating. The method described here will provide an efficient means to transfer multiple genes, in parallel, into cultured mammalian cells for high-throughput reverse genetics research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.