ArticleArchives of disease in childhood2005
Empyema: the use of broad range 16S rDNA PCR for pathogen detection.
Article in Archives of disease in childhood, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Comparison of Molecular Methods With Culture for Identifying Microbial Etiology in Pediatric Empyema Thoracis: A Systematic Review of Diagnostic Test Accuracy.Indian pediatrics · 2026Pooled it
- Evidence-Based Guideline on Prioritized Issues for the Management of Empyema Thoracis in Children.Indian pediatrics · 2026Guideline
- Liver abscess and septic shock due toFrontiers in medicine · 2025Article
- Severe pneumonia with empyema due to multiple anaerobic infections: case report and literature review.Frontiers in medicine · 2024Article
- Optimization and standardization of mNGS-based procedures for the diagnosis ofFrontiers in cellular and infection microbiology · 2023Article
- A challenging case of thoracic empyema caused byRespiratory medicine case reports · 2022Article
- Multiplex bacterial polymerase chain reaction in a cohort of patients with pleural effusion.BMC infectious diseases · 2020Observational
- The bacteriology in adult patients with pneumonia and parapneumonic effusions: increased yield with DNA sequencing method.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- An unusual case of seronegative, 16S PCR positiveJMM case reports · 2016Article
- Individuality, phenotypic differentiation, dormancy and 'persistence' in culturable bacterial systems: commonalities shared by environmental, laboratory, and clinical microbiology.F1000Research · 2015Review
- Real-time polymerase chain reaction for microbiological diagnosis of parapneumonic effusions in Canadian children.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Species-specific PCR improves detection of bacterial pathogens in parapneumonic empyema compared with 16S PCR and culture.The Pediatric infectious disease journal · 2013Article
- Community-Acquired Pneumonia in the Conjugate Vaccine Era.Journal of the Pediatric Infectious Diseases Society · 2012Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Molecular analysis improves pathogen identification and epidemiologic study of pediatric parapneumonic empyema.The Pediatric infectious disease journal · 2011Article
- Empyema thoracis: new insights into an old disease.European respiratory review : an official journal of the European Respiratory Society · 2010Review
- Usefulness of pneumococcal antigen detection in pleural effusion for the rapid diagnosis of infection by Streptococcus pneumoniae.European journal of pediatrics · 2010Article
- Empyema associated with community-acquired pneumonia: a Pediatric Investigator's Collaborative Network on Infections in Canada (PICNIC) study.BMC infectious diseases · 2008Article
- Duplex real-time PCR assay for detection of Streptococcus pneumoniae in clinical samples and determination of penicillin susceptibility.Journal of clinical microbiology · 2008Article
- Molecular analysis of human forearm superficial skin bacterial biota.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAn increase in the incidence of thoracic empyema in children has been reported. The causative pathogen is often unknown as pleural fluid is frequently sterile at the time of culture. The role of unusual organisms is unclear.
aims(1) To compare the detection of organisms in pleural fluid from children with empyema using a molecular technique (16S rDNA polymerase chain reaction (PCR)) and bacterial culture. (2) To compare the concordance of organisms identified using the two techniques and the influence of prior antibiotic treatment on positive detection rate.
methodsPleural fluid from children admitted with empyema between January 2000 and February 2002 was cultured and additionally analysed using broad range 16S rDNA PCR.
resultsPleural fluid was cultured from 32 patients, aged 1 month-16 years. Median duration of previous antibiotic therapy was 8 days (range 1-42 days). Six samples were culture positive and 22 were PCR positive. A causal organism was detected by PCR alone, after considering results from the local hospital, in 14 patients. There was complete concordance in organisms cultured and detected by PCR. Additional organisms detected by PCR were predominantly S pneumoniae, S pyogenes, and anaerobes.
conclusionsAnalysis of pleural fluid by broad range 16S rDNA PCR in addition to culture, increases organism identification in empyema.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.