ArticleJournal of virology2005
The red clover necrotic mosaic virus RNA2 trans-activator is also a cis-acting RNA2 replication element.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Article
- A trans-activator-like structure in RCNMV RNA1 evokes the origin of the trans-activator in RNA2.PLoS pathogens · 2020Article
- Review
- Modification of betanodavirus virulence by substitutions in the 3' terminal region of RNA2.The Journal of general virology · 2018Article
- Noncoding RNAs of Plant Viruses and Viroids: Sponges of Host Translation and RNA Interference Machinery.Molecular plant-microbe interactions : MPMI · 2016Review
- GAPDH--a recruits a plant virus movement protein to cortical virus replication complexes to facilitate viral cell-to-cell movement.PLoS pathogens · 2014Article
- Molecular biology and epidemiology of dianthoviruses.Advances in virus research · 2013Review
- Differential roles of Hsp70 and Hsp90 in the assembly of the replicase complex of a positive-strand RNA plant virus.Journal of virology · 2012Article
- Internal RNA Replication Elements are Prevalent in Tombusviridae.Frontiers in microbiology · 2012Article
- Review
- Template recognition mechanisms by replicase proteins differ between bipartite positive-strand genomic RNAs of a plant virus.Journal of virology · 2011Article
- Identification and characterization of the 480-kilodalton template-specific RNA-dependent RNA polymerase complex of red clover necrotic mosaic virus.Journal of virology · 2010Article
- The Red clover necrotic mosaic virus RNA-2 encoded movement protein is a second suppressor of RNA silencing.Virology · 2008Article
- A viral noncoding RNA generated by cis-element-mediated protection against 5'->3' RNA decay represses both cap-independent and cap-dependent translation.Journal of virology · 2008Article
- cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.Virology · 2008Article
- A multicomponent RNA-based control system regulates subgenomic mRNA transcription in a tombusvirus.Journal of virology · 2007Article
- Cap-independent translation mechanism of red clover necrotic mosaic virus RNA2 differs from that of RNA1 and is linked to RNA replication.Journal of virology · 2006Article
- A plant RNA virus suppresses RNA silencing through viral RNA replication.The EMBO journal · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the coat protein gene requires RNA-mediated trans-activation of subgenomic RNA synthesis in Red clover necrotic mosaic virus (RCNMV), the genome of which consists of two positive-strand RNAs, RNA1 and RNA2. The trans-acting RNA element required for subgenomic RNA synthesis from RNA1 has been mapped previously to the protein-coding region of RNA2, whereas RNA2 is not required for the replication of RNA1. In this study, we investigated the roles of the protein-coding region in RNA2 replication by analyzing the replication competence of RNA2 mutants containing deletions or nucleotide substitutions. Our results indicate that the same stem-loop structure (SL2) that functions as a trans-activator for RNA-mediated coat protein expression is critically required for the replication of RNA2 itself. Interestingly, however, disruption of the RNA-RNA interaction by nucleotide substitutions in the region of RNA1 corresponding to the SL2 loop of RNA2 does not affect RNA2 replication, indicating that the RNA-RNA interaction is not required for RNA2 replication. Further mutational analysis showed that, in addition to the stem-loop structure itself, nucleotide sequences in the stem and in the loop of SL2 are important for the replication of RNA2. These findings suggest that the structure and nucleotide sequence of SL2 in RNA2 play multiple roles in the virus life cycle.
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