ArticleRespiratory research2004
Effects of PM10 in human peripheral blood monocytes and J774 macrophages.
Article in Respiratory research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 72 citations in OpenAlex.
- Ingestion of titanium dioxide nanoparticles: a definite health risk for consumers and their progeny.Archives of toxicology · 2022Review
- Role of atmospheric particulate matter exposure in COVID-19 and other health risks in human: A review.Environmental research · 2021Review
- Effect of water-soluble PMJournal of environmental health science & engineering · 2021Article
- Exposure of CuO Nanoparticles Contributes to Cellular Apoptosis, Redox Stress, and Alzheimer's Aβ Amyloidosis.International journal of environmental research and public health · 2020Article
- Article
- Nanomaterials Versus Ambient Ultrafine Particles: An Opportunity to Exchange Toxicology Knowledge.Environmental health perspectives · 2017Review
- Holi colours contain PM10 and can induce pro-inflammatory responses.Journal of occupational medicine and toxicology (London, England) · 2016Article
- Cardiorespiratory treatments as modifiers of the relationship between particulate matter and health: a case-only analysis on hospitalized patients in Italy.Environmental research · 2015Article
- Variation in the composition and in vitro proinflammatory effect of urban particulate matter from different sites.Journal of biochemical and molecular toxicology · 2013Article
- Cytokine Production Modified by System X(c)- After PM10 and Asbestos Exposure.Journal of young investigators · 2012Article
- Exposure to moderate air pollution during late pregnancy and cord blood cytokine secretion in healthy neonates.PloS one · 2011Article
- Particulate air pollution, systemic oxidative stress, inflammation, and atherosclerosis.Air quality, atmosphere, & health · 2010Article
- Airborne particulate matter PM2.5 from Mexico City affects the generation of reactive oxygen species by blood neutrophils from asthmatics: an in vitro approach.Journal of occupational medicine and toxicology (London, England) · 2009Article
- Analysis of inflammatory markers in apparently healthy automobile vehicle drivers in response to exposure to traffic pollution fumes.Pakistan journal of medical sciencesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of PM10, one of the components of particulate air pollution, was investigated using human monocytes and a mouse macrophage cell line (J774). The study aimed to investigate the role of these nanoparticles on the release of the pro-inflammatory cytokine TNF-alpha and IL-1alpha gene expression. We also investigated the role of intracellular calcium signalling events and oxidative stress in control of these cytokines and the effect of the particles on the functioning of the cell cytoskeleton. We showed that there was an increase in intracellular calcium concentration in J774 cells on treatment with PM10 particles which could be significantly reduced with concomitant treatment with the calcium antagonists verapamil, the intracellular calcium chelator BAPTA-AM but not with the antioxidant nacystelyn or the calmodulin inhibitor W-7. In human monocytes, PM10 stimulated an increase in intracellular calcium which was reduced by verapamil, BAPTA-AM and nacystelyn. TNF-alpha release was increased with particle treatment in human monocytes and reduced by only verapamil and BAPTA-AM. IL-1alpha gene expression was increased with particle treatment and reduced by all of the inhibitors. There was increased F-actin staining in J774 cells after treatment with PM10 particles, which was significantly reduced to control levels with all the antagonists tested. The present study has shown that PM10 particles may exert their pro-inflammatory effects by modulating intracellular calcium signalling in macrophages leading to expression of pro-inflammatory cytokines. Impaired motility and phagocytic ability as shown by changes in the F-actin cytoskeleton is likely to play a key role in particle clearance from the lung.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.