ArticleThe Biochemical journal2005
A new class of glutathione S-transferase from the hepatopancreas of the red sea bream Pagrus major.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 76 citations in OpenAlex.
- Testing the Capability of Embedding-Based Alignments on the GST Superfamily Classification: The Role of Protein Length.Molecules (Basel, Switzerland) · 2024Article
- Integrating UPLC-Q-Orbitrap MS with serum pharmacochemistry network and experimental verification to explore the pharmacological mechanisms ofFrontiers in pharmacology · 2024Article
- Comparison of the Effects of 5-Hydroxymethylfurfural in Milk Powder Matrix and Standard Water on Oxidative Stress System of Zebrafish.Foods (Basel, Switzerland) · 2022Article
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- Roles of Two Glutathione-Dependent 3,6-Dichlorogentisate Dehalogenases in Rhizorhabdus dicambivorans Ndbn-20 in the Catabolism of the Herbicide Dicamba.Applied and environmental microbiology · 2018Article
- Dual NRF2 paralogs in Coho salmon and their antioxidant response element targets.Redox biology · 2016Article
- The Association between Gene-Environment Interactions and Diseases Involving the Human GST Superfamily with SNP Variants.International journal of environmental research and public health · 2016Review
- Glutathione S-transferase activities in African catfish injected with β-naphthoflavone: effects of ploidy, gender, dose, and sampling time.Environmental monitoring and assessment · 2015Article
- Cloning, expression and analysis of the olfactory glutathione S-transferases in coho salmon.Biochemical pharmacology · 2013Article
- Purification and properties of glutathione S-transferase from the pike liver.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2013Article
- Effect of cadmium on glutathione S-transferase and metallothionein gene expression in coho salmon liver, gill and olfactory tissues.Aquatic toxicology (Amsterdam, Netherlands) · 2012Article
- Transcriptome sequencing and microarray development for the Manila clam, Ruditapes philippinarum: genomic tools for environmental monitoring.BMC genomics · 2011Article
- Molecular mechanisms of microcystin toxicity in animal cells.International journal of molecular sciences · 2010Review
- Crystallization and preliminary X-ray crystallographic studies of the rho-class glutathione S-transferase from the Antarctic clam Laternula elliptica.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Crystallization and preliminary X-ray diffraction analysis of a glutathione S-transferase from Xylella fastidiosa.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Characterization of hepatic glutathione S-transferases in coho salmon (Oncorhynchus kisutch).Aquatic toxicology (Amsterdam, Netherlands) · 2007Article
- Marine glutathione S-transferases.Marine biotechnology (New York, N.Y.)Review
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate drug deposition and metabolism in cultured marine fishes, in a previous study we isolated and purified the GSTs (glutathione S-transferases) from the hepatopancreas of the red sea bream Pagrus major that contained 25 and 28 kDa GST subunits. The 25 kDa GST subunits encoded by two genes (GSTA1 and GSTA2) have been identified as Alpha-class GSTs. In the present study, we performed the molecular cloning and characterization of the GSTR1 gene encoding the 28 kDa GST subunit from the Pa. major hepatopancreas. The nucleotide sequence of GSTR1 was composed of an ORF (open reading frame) of 675 bp encoding a protein of 225 residues with a predicted molecular mass of 25.925 Da. A search of the BLAST protein database revealed that the deduced amino acid sequence of GSTR1 was structurally similar to that of GSTs derived from other fishes such as largemouth bass (Micropterus salmoides) and plaice (Pleuronectes platessa). The genomic DNA containing the GSTR1 gene was found to consist of six exons and five introns quite distinct from mammalian Theta-class GSTs. We have purified and characterized the recombinant GSTR1 enzyme (pmGSTR1-1) which showed activity only towards 1-chloro-2,4-dinitrobenzene, although it had no detectable activity towards cumene hydroperoxide, 1,2-dichloro-4-nitrobenzene, ethacrynic acid, 4-hydroxynonenal and p-nitrobenzyl chloride. Moreover, pmGSTR1-1 revealed remarkable heat instability (melting temperature Tm=30.3+/-0.11 degrees C). Collectively, our results indicated that the characteristic GST genes including GSTR1 have been conserved and functional in fishes. Therefore we designate them 'Rho-class', a new class of GSTs.
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