Evidence map›Paper›PMID 15609514›Full record

ReviewCurrent topics in microbiology and immunology2005

Development of mouse hepatitis virus and SARS-CoV infectious cDNA constructs.

R S Baric, A C Sims

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Current topics in microbiology and immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
7.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 31 citations in OpenAlex.

  1. Ayurvedic and Chinese Herbs against Coronaviruses.Current pharmaceutical design · 2024
    Review
  2. Review
  3. COVID-19 is a natural infectious disease.Journal of biosafety and biosecurity · 2022
    Review
  4. A call for an independent inquiry into the origin of the SARS-CoV-2 virus.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Shrimp molecular responses to viral pathogens.Marine biotechnology (New York, N.Y.) · 2011
    Review
  11. Coronavirus pathogenesis.Advances in virus research · 2011
    Review
  12. A mechanism of virus-induced demyelination.Interdisciplinary perspectives on infectious diseases · 2010
    Article
  13. The role of programmed-1 ribosomal frameshifting in coronavirus propagation.Frontiers in bioscience : a journal and virtual library · 2008
    Review
  14. Review
  15. Review
  16. Review
  17. SARS CoV replication and pathogenesis in human airway epithelial cultures.Advances in experimental medicine and biology · 2006
    Article
  18. The molecular biology of coronaviruses.Advances in virus research · 2006
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

R S BaricDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7400, USA. rbaric@email.unc.edu
A C Sims
University of North Carolina at Chapel Hill · USUniversity of North Carolina Health Care · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genomes of transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV) have been generated with a novel construction strategy that allows for the assembly of very large RNA and DNA genomes from a panel of contiguous cDNA subclones. Recombinant viruses generated from these methods contained the appropriate marker mutations and replicated as efficiently as wild-type virus. The MHV cloning strategy can also be used to generate recombinant viruses that contain foreign genes or mutations at virtually any given nucleotide. MHV molecular viruses were engineered to express green fluorescent protein (GFP), demonstrating the feasibility of the systematic assembly approach to create recombinant viruses expressing foreign genes. The systematic assembly approach was used to develop an infectious clone of the newly identified human coronavirus, the serve acute respiratory syndrome virus (SARS-CoV). Our cloning and assembly strategy generated an infectious clone within 2 months of identification of the causative agent of SARS, providing a critical tool to study coronavirus pathogenesis and replication. The availability of coronavirus infectious cDNAs heralds a new era in coronavirus genetics and genomic applications, especially within the replicase proteins whose functions in replication and pathogenesis are virtually unknown.

Indexed as

Genome, ViralRecombination, GeneticAnimalsBase SequenceDNA, ComplementaryHumansMiceMolecular Sequence DataMurine hepatitis virusSevere acute respiratory syndrome-related coronavirusTransmissible gastroenteritis virusDNA, Complementary

Identifiers

PMID15609514
PMCPMC7122489
OpenAlexW2202123579

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.