ArticleWorld journal of gastroenterology2005
Heat shock protein 70 antisense oligonucleotide inhibits cell growth and induces apoptosis in human gastric cancer cell line SGC-7901.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 48 citations in OpenAlex.
- HspBP1 is a dual function regulatory protein that controls both DNA repair and apoptosis in breast cancer cells.Cell death & disease · 2022Article
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- Fas expression is downregulated in gastric cancer.Molecular medicine reports · 2017Article
- VEGF Overexpression Predicts Poor Survival in Hepatocellular Carcinoma.Open medicine (Warsaw, Poland) · 2017Article
- Downregulation of Sp1 by Minnelide leads to decrease in HSP70 and decrease in tumor burden of gastric cancer.PloS one · 2017Article
- Hyperthermia combined with 5-fluorouracil promoted apoptosis and enhanced thermotolerance in human gastric cancer cell line SGC-7901.OncoTargets and therapy · 2015Article
- Prognostic significance of heat shock protein 70 expression in early gastric carcinoma.Korean journal of pathology · 2013Article
- Artificial box C/D RNAs affect pre-mRNA maturation in human cells.BioMed research international · 2013Article
- Effects of HSP70 antisense oligonucleotide on the proliferation and apoptosis of human hepatocellular carcinoma cells.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2010Article
- An approach to the study of gene expression in hepatocarcinogenesis initiation.Translational oncology · 2010Article
- Association of HSP70-hom genetic variant with prostate cancer risk.Molecular biology reports · 2008Article
- Heat shock proteins in animal neoplasms and human tumours--a comparison.Cell stress & chaperones · 2008Review
- Heat shock proteins: essential proteins for apoptosis regulation.Journal of cellular and molecular medicine · 2008Review
- HSP70s: From Tumor Transformation to Cancer Therapy.Clinical medicine. Oncology · 2008Article
- Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer.World journal of gastroenterology · 2006Article
- Expressions of HSP70 and HSP27 in hepatocellular carcinoma.Journal of Korean medical science · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimHeat shock protein (HSP)70 is over-expressed in human gastric cancer and plays an important role in the progression of this cancer. We investigated the effects of antisense HSP70 oligomer on human gastric cancer cell line SGC-7901, and its potential role in gene therapy for this cancer.
methodsHuman gastric cancer cell line SGC-7901 was treated in vitro with various concentrations of antisense HSP70 oligonucleotides at different intervals. Growth inhibition was determined as percentage by trypan blue dye exclusion test. Extracted DNA was electrophoresed on agarose gel, and distribution of cell cycle and kinetics of apoptosis induction were analyzed by propidium iodide DNA incorporation using flow cytometry, which was also used to detect the effects of antisense oligomer pretreatment on the subsequent apoptosis induced by heat shock in SGC-7901 cells. Proteins were extracted for simultaneous measurement of HSP70 expression level by SDS-PAGE Western blotting.
resultsThe number of viable cells decreased in a dose- and time-dependent manner, and ladder-like patterns of DNA fragments were observed in SGC-7901 cells treated with antisense HSP70 oligomers at a concentration of 10 mumol/L for 48 h or 8 micromol/L for 72 h, which were consistent with inter-nucleosomal DNA fragmentation. Flow cytometric analysis showed a dose- and time-dependent increase in apoptotic rate by HSP70 antisense oligomers. This response was accompanied with a decrease in the percentage of cells in the G1 and S phases of the cell cycle, suggesting inhibition of cell proliferation. In addition, flow cytometry also showed that pretreatment of SGC-7901 cells with HSP70 antisense oligomers enhanced the subsequent apoptosis induced by heat shock treatment. Western blotting demonstrated that HSP70 antisense oligomers inhibited HSP70 expression, which preceded apoptosis, and HSP70 was undetectable at the concentration of 10 micromol/L for 48 h or 8 micromol/L for 72 h.
conclusionAntisense HSP70 oligomers can abrogate HSP70 expression in SGC-7901 cells, which may in turn induce apoptosis and inhibit cell proliferation, conversely suggesting that HSP70 is required for the proliferation and survival of human gastric cancer cells under normal conditions.
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