ArticleNature biotechnology2005
Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes.
Article in Nature biotechnology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed.
- Metagenomics and its impact on environmental and therapeutic microbiology.Archives of microbiology · 2025Review
- Use of genomics & proteomics in studying lipase producing microorganisms & its application.Food chemistry. Molecular sciences · 2024Article
- Applications and Tuning Strategies for Transcription Factor-Based Metabolite Biosensors.Biosensors · 2023Review
- Metagenomic discovery of lipases with predicted structural similarity to Candida antarctica lipase B.PloS one · 2023Article
- Strategies for Natural Products Discovery from Uncultured Microorganisms.Molecules (Basel, Switzerland) · 2021Review
- Construction of Aerobic/Anaerobic-Substrate-Induced Gene Expression Procedure for Exploration of Metagenomes From Subseafloor Sediments.Frontiers in microbiology · 2021Article
- Advances in Metagenomics and Its Application in Environmental Microorganisms.Frontiers in microbiology · 2021Review
- A novel bacterial carboxylesterase identified in a metagenome derived-clone from Brazilian mangrove sediments.Molecular biology reports · 2020Article
- A progesterone biosensor derived from microbial screening.Nature communications · 2020Article
- Bioprospecting Microbial Diversity for Lignin Valorization: Dry and Wet Screening Methods.Frontiers in microbiology · 2020Review
- Rapid Enrichment and Isolation of Polyphosphate-Accumulating Organisms Through 4'6-Diamidino-2-Phenylindole (DAPI) Staining With Fluorescence-Activated Cell Sorting (FACS).Frontiers in microbiology · 2020Article
- Functional screening of a soil metagenome for DNA endonucleases by acquired resistance to bacteriophage infection.Molecular biology reports · 2020Article
- De novo design of programmable inducible promoters.Nucleic acids research · 2019Article
- Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives.Molecular biotechnology · 2019Review
- Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- An Alternative Platform for Protein Expression Using an Innate Whole Expression Module from Metagenomic DNA.Microorganisms · 2019Article
- Genetically encoded biosensors for lignocellulose valorization.Biotechnology for biofuels · 2019Review
- Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.BioMed research international · 2019Review
- Recent Advances in Function-based Metagenomic Screening.Genomics, proteomics & bioinformatics · 2018Review
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
23 more citing papers are in PubMed but not listed here.
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Recent awareness that most microorganisms in the environment are resistant to cultivation has prompted scientists to directly clone useful genes from environmental metagenomes. Two screening methods are currently available for the metagenome approach, namely, nucleotide sequence-based screening and enzyme activity-based screening. Here we have introduced and optimized a third option for the isolation of novel catabolic operons, that is, substrate-induced gene expression screening (SIGEX). This method is based on the knowledge that catabolic-gene expression is generally induced by relevant substrates and, in many cases, controlled by regulatory elements situated proximate to catabolic genes. For SIGEX to be high throughput, we constructed an operon-trap gfp-expression vector available for shotgun cloning that allows for the selection of positive clones in liquid cultures by fluorescence-activated cell sorting. The utility of SIGEX was demonstrated by the cloning of aromatic hydrocarbon-induced genes from a groundwater metagenome library and subsequent genome-informatics analysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.