ArticleProtein science : a publication of the Protein Society2005
Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli.
Article in Protein science : a publication of the Protein Society, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 97 citations in OpenAlex.
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- Analysis of the Antigenic Properties of Membrane Proteins of Mycobacterium tuberculosis.Scientific reports · 2019Article
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- A statistical model for improved membrane protein expression using sequence-derived features.The Journal of biological chemistry · 2018Article
- Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.Bioprocess and biosystems engineering · 2018Article
- Protein-Specific Features Associated with Variability in Human Antibody Responses toThe American journal of tropical medicine and hygiene · 2018Article
- Improving membrane protein expression by optimizing integration efficiency.The Journal of biological chemistry · 2017Article
- Construction and immunogenicity of a new Fc-based subunit vaccine candidate against Mycobacterium tuberculosis.Molecular biology reports · 2016Article
- Structure of CrgA, a cell division structural and regulatory protein from Mycobacterium tuberculosis, in lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Self-assembled MmsF proteinosomes control magnetite nanoparticle formation in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Exploration of novel cellular and serological antigen biomarkers in the ORFeome of Mycobacterium tuberculosis.Molecular & cellular proteomics : MCP · 2014Article
- Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples.Nature protocols · 2013Article
- Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris.Protein science : a publication of the Protein Society · 2013Article
- Mycobacterium tuberculosis CwsA interacts with CrgA and Wag31, and the CrgA-CwsA complex is involved in peptidoglycan synthesis and cell shape determination.Journal of bacteriology · 2012Article
- Mistic and TarCF as fusion protein partners for functional expression of the cannabinoid receptor 2 in Escherichia coli.Protein expression and purification · 2012Article
- A systematic assessment of mature MBP in membrane protein production: overexpression, membrane targeting and purification.Protein expression and purification · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Seventy integral membrane proteins from the Mycobacterium tuberculosis genome have been cloned and expressed in Escherichia coli. A combination of T7 promoter-based vectors with hexa-His affinity tags and BL21 E. coli strains with additional tRNA genes to supplement sparsely used E. coli codons have been most successful. The expressed proteins have a wide range of molecular weights and number of transmembrane helices. Expression of these proteins has been observed in the membrane and insoluble fraction of E. coli cell lysates and, in some cases, in the soluble fraction. The highest expression levels in the membrane fraction were restricted to a narrow range of molecular weights and relatively few transmembrane helices. In contrast, overexpression in insoluble aggregates was distributed over a broad range of molecular weights and number of transmembrane helices.
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