ArticleVeterinary microbiology2005
An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces.
Article in Veterinary microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Spatiotemporal distribution and molecular characteristics of porcine diarrhea-associated viruses detected in Chinese pigs.Virulence · 2026Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Complexity of Diarrhea-Associated Viruses in Stunted Pigs Identified by Viral Metagenomics.Transboundary and emerging diseases · 2025Article
- Current State of Molecular and Serological Methods for Detection of Porcine Epidemic Diarrhea Virus.Pathogens (Basel, Switzerland) · 2022Review
- Development of a Novel Double Antibody Sandwich Quantitative Enzyme-Linked Immunosorbent Assay for Detection of Porcine Epidemic Diarrhea Virus Antigen.Frontiers in veterinary science · 2020Article
- Dual priming oligonucleotide (DPO)-based real-time RT-PCR assay for accurate differentiation of four major viruses causing porcine viral diarrhea.Molecular and cellular probes · 2019Article
- Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method.BMC veterinary research · 2019Article
- Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3.Molecular and cellular probes · 2019Article
- A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay.BMC veterinary research · 2019Article
- Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety.Reviews in medical virology · 2019Review
- Porcine epidemic diarrhea virus: An overview of current virological and serological diagnostic methods.Virus research · 2016Review
- A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay.Journal of virological methods · 2015Article
- Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus.BMC veterinary research · 2015Article
- Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus.Journal of virological methods · 2015Article
- Emergence of porcine epidemic diarrhea virus in southern Germany.BMC veterinary research · 2015Article
- Porcine epidemic diarrhea: a review of current epidemiology and available vaccines.Clinical and experimental vaccine research · 2015Review
- Development of an antigen capture enzyme-linked immunosorbent assay for virus detection based on porcine epidemic diarrhea virus monoclonal antibodies.Viral immunology · 2015Article
- Porcine epidemic diarrhoea: new insights into an old disease.Porcine health management · 2015Article
- Probability of introducing porcine epidemic diarrhea virus into Danish pig herds by imported spray-dried porcine plasma.Porcine health management · 2015Article
- Detection of immunoglobulin (Ig) A antibodies against porcine epidemic diarrhea virus (PEDV) in fecal and serum samples.MethodsX · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.