ArticleCurrent genetics2005
Isolation and characterisation of three moss-derived beta-tubulin promoters suitable for recombinant expression.
Article in Current genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 36 citations in OpenAlex.
- Differential GTP-dependent in-vitro polymerization of recombinant Physcomitrella FtsZ proteins.Scientific reports · 2025Article
- Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella.Plant cell reports · 2024Article
- Process Engineering of Biopharmaceutical Production in Moss BioreactorsFrontiers in bioengineering and biotechnology · 2022Article
- Physcomitrella patens, a versatile synthetic biology chassis.Plant cell reports · 2018Review
- Combination of the EndogenousFrontiers in plant science · 2017Article
- Moss-made pharmaceuticals: from bench to bedside.Plant biotechnology journal · 2015Review
- Glycoprotein production in moss bioreactors.Plant cell reports · 2012Review
- Controlled expression of recombinant proteins in Physcomitrella patens by a conditional heat-shock promoter: a tool for plant research and biotechnology.Plant molecular biology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moss Physcomitrella patens is an excellent tool to study plant gene-function relationships due to its high rate of homologous recombination (HR). It has also been shown to be very useful in the production of recombinant proteins which are secreted into a simple medium. Thus, there is a need for suitable promoters functional in this well established model organism. We isolated genomic flanking regions of the beta-tubulin gene family from Physcomitrella, concentrating on those family members showing high transcript abundance integrated over gametophytic tissues. Using a novel, fast and reliable quantification assay based on the transient expression and secretion of a recombinant human protein, three genomic upstream regions were characterised in serial deletion constructs. Expression rates were up to three times higher than those obtained with the 35S cauliflower mosaic virus (35S) promoter, which served as a reference.
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Registered trials
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