ArticlePlanta2004
Glucosylation of the saffron apocarotenoid crocetin by a glucosyltransferase isolated from Crocus sativus stigmas.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 147 citations in OpenAlex.
- Temporal dynamics of ruminal metabolome and fermentation in response to molasses-treated Guinea grass-cowpea silage.Veterinary and animal science · 2026Article
- Natural CCD2 Variants and RNA Interference for Boosting Crocin Biosynthesis in Tomato.Biology · 2025Article
- Water-soluble carotenoid: focused on natural carotenoid crocin.Food science and biotechnology · 2025Review
- Advanced Technologies for Large Scale Supply of Marine Drugs.Marine drugs · 2025Review
- Potential therapeutic effects of crocin.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Co-regulatory network analysis of the main secondary metabolite (SM) biosynthesis in Crocus sativus L.Scientific reports · 2024Article
- Exploring the therapeutic efficacy of crocetin in oncology: an evidence-based review.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Research progress on the pharmacological activity, biosynthetic pathways, and biosynthesis of crocins.Beilstein journal of organic chemistry · 2024Review
- Research Progress in Heterologous Crocin Production.Marine drugs · 2023Review
- Efficacy of Hydroponically Cultivated Saffron in the Preservation of Retinal Pigment Epithelium.Molecules (Basel, Switzerland) · 2023Article
- Terpenoid Transport in Plants: How Far from the Final Picture?Plants (Basel, Switzerland) · 2023Review
- Redox and Hormonal Changes in the Transcriptome of Grape (Plants (Basel, Switzerland) · 2022Article
- Engineering high levels of saffron apocarotenoids in tomato.Horticulture research · 2022Article
- Metabolic Engineering of Crocin Biosynthesis inFrontiers in plant science · 2022Article
- The Pharmacological Activities ofOxidative medicine and cellular longevity · 2022Review
- Fortification and bioaccessibility of saffron apocarotenoids in potato tubers.Frontiers in nutrition · 2022Article
- A New Glycosyltransferase Enzyme from Family 91, UGT91P3, Is Responsible for the Final Glucosylation Step of Crocins in Saffron (International journal of molecular sciences · 2021Article
- Specific decorations of 17-hydroxygeranyllinalool diterpene glycosides solve the autotoxicity problem of chemical defense in Nicotiana attenuata.The Plant cell · 2021Article
- The effect of salt stress on the production of apocarotenoids and the expression of genes related to their biosynthesis in saffron.Molecular biology reports · 2021Article
- Crocetin: A Systematic Review.Frontiers in pharmacology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saffron, the dry stigma of Crocus sativus L., is considered to be the world's most expensive spice. Three major apocarotenoids--crocin, crocetin and picrocrocin--are responsible for the colour and bitter taste of saffron. The final step in the biosynthesis of the 20-carbon esterified carotenoid crocin is the transformation of the insoluble crocetin into a soluble and stable storage form by glucosylation. These glucosylation reactions are catalysed by glucosyltransferases (GTases) that play a crucial role in natural-product biosynthesis. Using degenerate primers designed to match the plant secondary product GTase (PSPG) box we cloned two cDNAs, UGTCs2 and UGTCs3, from C. sativus stigmas that encode putative polypeptides of 460 and 475 amino acids, respectively. These genes were expressed differentially in saffron tissues. UGTCs2 was mainly expressed in fully developed stigmas, whereas UGTCs3 was mainly expressed in stamens. The UGTCs2 transcript was not detected in the stigma tissue of a Crocus species that does not synthesize crocin, while UGTCs3 and other structural genes for carotenoid biosynthesis were expressed in the stigma of all tested Crocus species. To identify the biochemical function of UGTCs2, the isolated cDNA was expressed in Escherichia coli cells. The recombinant protein UGTCs2 had glucosylation activity against crocetin, crocetin beta-D-glucosyl ester and crocetin beta-D-gentibiosyl ester. These results might suggest that the isolated clone UGTCs2 codes for a saffron crocetin GTase.
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