ArticlePlant molecular biology2004
PVX-Cre-mediated marker gene elimination from transgenic plants.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Improving transient expression inFrontiers in plant science · 2024Article
- Clean gene technology to develop selectable marker-free pod borer-resistant transgenic pigeon pea events involving the constitutive expression of Cry1Ac.Applied microbiology and biotechnology · 2022Article
- Article
- An Efficient Gene Excision System in Maize.Frontiers in plant science · 2020Article
- Enhanced foreign protein accumulation in Nicotiana benthamiana leaves co-infiltrated with a TMV vector and plant cell cycle regulator genes.Transgenic research · 2019Article
- Transient plant production ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Utilization of PVX-Cre expression vector in potato.Transgenic research · 2012Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Development of a simple and efficient system for excising selectable markers in Arabidopsis using a minimal promoter::Cre fusion construct.Molecules and cells · 2012Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Zinc finger nuclease-mediated transgene deletion.Plant molecular biology · 2010Article
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- Developmentally regulated site-specific marker gene excision in transgenic B. napus plants.Plant cell reports · 2009Article
- Excision of a selective marker in transgenic rice using a novel Cre/loxP system controlled by a floral specific promoter.Transgenic research · 2008Article
- Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.Plant cell reports · 2008Article
- Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana.Transgenic research · 2008Article
- Marker-free transgenic plants through genetically programmed auto-excision.Plant physiology · 2007Article
- Stable recombinase-mediated cassette exchange in Arabidopsis using Agrobacterium tumefaciens.Plant physiology · 2007Article
- Reactivation of an integrated disabled viral vector using a Cre-loxP recombination system in Arabidopsis thaliana.Transgenic research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cre recombinase gene from bacteriophage P1 was transiently expressed by a Potato Virus X (PVX)-based vector in transgenic lox -target Nicotiana benthamiana plants to remove the selectable marker gene. The target construct consisted of two directly oriented lox sites flanking a bar gene located between a gfp coding region and an upstream CaMV 35S promoter. The Cre-mediated excision of intervening sequence placed the gfp coding region under the transcriptional control of the CaMV 35S promoter. GFP activity was observed in PVX-Cre systemically infected leaves, regenerants from PVX-Cre infected explants and T1 progeny of these regenerants. PVX-Cre was removed efficiently from the regenerants by adding the nucleoside analogue ribavirin to the culture medium. Molecular data proved a correlation between gfp expression and precise site-specific excision of the bar gene in all examined transgenic lines. The frequency of recombination expressed as a percentage of regenerated plants exhibiting marker gene excision varied from 48% to 82%. These results demonstrate that a plant virus vector can be used efficiently to express cre recombinase in vivo providing an alternative method for the production of transgenic plants without marker genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.