ArticlePlant molecular biology2004
Generation of fertile transplastomic soybean.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 194 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Article
- Stable plastid transformation in kiwifruit (aBIOTECH · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Plastid dsRNA transgenes trigger phased small RNA-based gene silencing of nuclear-encoded genes.The Plant cell · 2023Article
- Plastid engineering using episomal DNA.Plant cell reports · 2023Review
- Overexpression of soybean trypsin inhibitor genes decreases defoliation by corn earworm (Frontiers in plant science · 2023Article
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Insights into plastome ofSaudi journal of biological sciences · 2022Article
- The chloroplast genome ofFrontiers in plant science · 2022Article
- Advancing organelle genome transformation and editing for crop improvement.Plant communications · 2021Review
- Green giant-a tiny chloroplast genome with mighty power to produce high-value proteins: history and phylogeny.Plant biotechnology journal · 2021Review
- Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinase.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Plastome engineering in vegetable crops: current status and future prospects.Molecular biology reports · 2020Review
- Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.Plant biotechnology journal · 2019Article
- Construction of a species-specific vector for improved plastid transformation efficiency in3 Biotech · 2019Article
- Efficient Plastid Transformation in Arabidopsis.Plant physiology · 2017Article
- Construction of Plastid Expression Vector and Development of Genetic Transformation System for the Seaweed Pyropia yezoensis.Marine biotechnology (New York, N.Y.) · 2017Article
- Chloroplast genomes: diversity, evolution, and applications in genetic engineering.Genome biology · 2016Review
- The Engineered Chloroplast Genome Just Got Smarter.Trends in plant science · 2015Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here the development of a plastid transformation method for soybean, a leguminous plant of major agronomic interest. Chloroplasts from embryogenic tissue of Glycine max have been successfully transformed by bombardment. The transforming DNA carries a spectinomycin resistance gene (aadA) under the control of tobacco plastid regulatory expression elements, flanked by two adjacent soybean plastome sequences allowing its targeted insertion between the trnV gene and the rps12/7 operon. All generated spectinomycin resistant plants were transplastomic and no remaining wild type plastome copies were detected. No spontaneous mutants were obtained. The transformation efficiency is similar to that of tobacco plastids. All transplastomic T0 plants were fertile and T1 progeny was uniformly spectinomycin resistant, showing the stability of the plastid transgene. This is the first report on the generation of fertile transplastomic soybean.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.