ArticlePlant molecular biology2004
Interaction of maize Opaque-2 and the transcriptional co-activators GCN5 and ADA2, in the modulation of transcriptional activity.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Transcriptional adapter ADA2 regulates yield and end-use quality through liquid-liquid phase separation in wheat.Journal of integrative plant biology · 2026Article
- Epigenetics and its role in effecting agronomical traits.Frontiers in plant science · 2022Review
- Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.The Plant cell · 2018Article
- Maize opaque mutants are no longer so opaque.Plant reproduction · 2018Review
- Uniparental and transgressive expression of α-zeins in maize endosperm of o2 hybrid lines.PloS one · 2018Article
- Rice Homeodomain Protein WOX11 Recruits a Histone Acetyltransferase Complex to Establish Programs of Cell Proliferation of Crown Root Meristem.The Plant cell · 2017Article
- Diversification of the Histone Acetyltransferase GCN5 through Alternative Splicing inFrontiers in plant science · 2017Article
- Stress-MediatedFrontiers in plant science · 2016Review
- Genome-wide characterization of cis-acting DNA targets reveals the transcriptional regulatory framework of opaque2 in maize.The Plant cell · 2015Article
- Composition of the SAGA complex in plants and its role in controlling gene expression in response to abiotic stresses.Frontiers in plant science · 2015Review
- Zea mays Taxilin protein negatively regulates opaque-2 transcriptional activity by causing a change in its sub-cellular distribution.PloS one · 2012Article
- Negative regulation of defence signalling pathways by the EDR1 protein kinase.Molecular plant pathology · 2011Article
- The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses.BMC genomics · 2011Article
- Chromatin and DNA modifications in the Opaque2-mediated regulation of gene transcription during maize endosperm development.The Plant cell · 2009Article
- Combination of novel green fluorescent protein mutant TSapphire and DsRed variant mOrange to set up a versatile in planta FRET-FLIM assay.Plant physiology · 2008Article
- Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension.The Plant cell · 2007Article
- Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site.Plant physiology · 2007Article
- Isolation and characterization of a GCN5-interacting protein from Arabidopsis thaliana.Planta · 2007Article
- The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods.Plant methods · 2006Article
- Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity.The Plant cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize Opaque-2 (ZmO2), a bZip class transcription factor has been shown to activate the transcription of a series of genes expressed in the maturation phase of endosperm development. Activation requires the presence of one or more enhancer binding sites, which confer the propensity for activation by ZmO2 on heterologous promoters and in heterologous plant cell types, such as tobacco mesophyll protoplasts. The region of ZmO2 required for conferring transcriptional activation has been localised to a stretch of acidic residues in the N-terminal portion of the ZmO2 sequence, which is conserved between O2-related bZip factor sequences. Previously we identified the maize homologues of yeast transcriptional co-activators GCN5 and ADA2 that are implicated in nucleosome modification and transcription. In the present study we have shown that transcriptional modulation by ZmO2 involves the intranuclear interaction of ZmO2 with ZmADA2 and ZmGCN5. Förster resonance energy transfer (FRET) based techniques have enabled us to estimate the intracellular site of these intermolecular interactions. As a functional readout of these intranuclear interactions, we used the ZmO2 responsive maize b-32 promoter to drive the beta-glucuronidase (GUS) in the presence and absence of ZmGCN5 and ZmADA2. Our results suggest that the likely recruitment of ZmADA2 and ZmGCN5 modulates the transactivation of b-32 promoter by ZmO2 and that there may be a competition between ZmGCN5 and ZmO2 for binding to the amino-terminal of ZmADA2. The results may be taken as a paradigm for other processes of transcriptional modulation in planta involving acidic activation domains.
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