ArticlePlant molecular biology2004
Gamma carbonic anhydrases in plant mitochondria.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 132 citations in OpenAlex.
- Article
- A Novel Strategy for Highly Efficient Heterologous Expression of Carbonic Anhydrase inInternational journal of molecular sciences · 2026Article
- Mitochondrial β-carbonic anhydrase is a conserved metabolic rheostat for branched-chain amino acid catabolism and metabolic flexibility.The New phytologist · 2026Article
- Genome Study of α-, β-, and γ-Carbonic Anhydrases from the Thermophilic Microbiome of Marine Hydrothermal Vent Ecosystems.Biology · 2023Article
- α-CAs from Photosynthetic Organisms.International journal of molecular sciences · 2022Review
- Contribution of the Mitochondrial Carbonic Anhydrase (MoCA1) to Conidiogenesis and Pathogenesis inFrontiers in microbiology · 2022Article
- A ferredoxin bridge connects the two arms of plant mitochondrial complex I.The Plant cell · 2021Article
- In Silico Investigation of Potential Applications of Gamma Carbonic Anhydrases as Catalysts of COInternational journal of molecular sciences · 2021Article
- Advances in understanding the physiological role and locations of carbonic anhydrases in C3 plant cells.Protoplasma · 2021Review
- Molecular cloning and transcriptional regulation of two γ-carbonic anhydrase genes in the green macroalga Ulva prolifera.Genetica · 2021Article
- Specific features and assembly of the plant mitochondrial complex I revealed by cryo-EM.Nature communications · 2020Article
- Functional redundancy and divergence of β-carbonic anhydrases in Physcomitrella patens.Planta · 2020Article
- Different Types Domains are Present in Complex I from Immature Seeds and of CA Adult Plants in Arabidopsis thaliana.Plant & cell physiology · 2019Article
- Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana.Plant cell reports · 2019Article
- Transport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead.International journal of molecular sciences · 2018Review
- An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.Metabolites · 2018Review
- The diversity of CO2-concentrating mechanisms in marine diatoms as inferred from their genetic content.Journal of experimental botany · 2017Article
- Plant Carbonic Anhydrases: Structures, Locations, Evolution, and Physiological Roles.Molecular plant · 2017Review
- The CA domain of the respiratory complex I is required for normal embryogenesis in Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Deficient plastidic fatty acid synthesis triggers cell death by modulating mitochondrial reactive oxygen species.Cell research · 2015Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three genes from Arabidopsis thaliana with high sequence similarity to gamma carbonic anhydrase (gammaCA), a Zn containing enzyme from Methanosarcina thermophila (CAM), were identified and characterized. Evolutionary and structural analyses predict that these genes code for active forms of gammaCA. Phylogenetic analyses reveal that these Arabidopsis gene products cluster together with CAM and related sequences from alpha and gamma proteobacteria, organisms proposed as the mitochondrial endosymbiont ancestor. Indeed, in vitro and in vivo experiments indicate that these gene products are transported into the mitochondria as occurs with several mitochondrial protein genes transferred, during evolution, from the endosymbiotic bacteria to the host genome. Moreover, putative CAM orthologous genes are detected in other plants and green algae and were predicted to be imported to mitochondria. Structural modeling and sequence analysis performed in more than a hundred homologous sequences show a high conservation of functionally important active site residues. Thus, the three histidine residues involved in Zn coordination (His 81, 117 and 122), Arg 59, Asp 61, Gin 75, and Asp 76 of CAM are conserved and properly arranged in the active site cavity of the models. Two other functionally important residues (Glu 62 and Glu 84 of CAM) are lacking, but alternative amino acids that might serve to their roles are postulated. Accordingly, we propose that photosynthetic eukaryotic organisms (green algae and plants) contain gammaCAs and that these enzymes codified by nuclear genes are imported into mitochondria to accomplish their biological function.
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