ArticlePlant molecular biology2004
Maize recombinant non-C4 NADP-malic enzyme: a novel dimeric malic enzyme with high specific activity.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Integrative crystallography and molecular dynamics reveal isoform-specific ligand interaction networks in C4 and nonC4 plant NADP-malic enzyme.The Plant journal : for cell and molecular biology · 2026Article
- Substrate Promiscuity ofJACS Au · 2024Article
- Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits.The Plant cell · 2022Article
- Independent Recruitment of Duplicated β-Subunit-Coding NAD-ME Genes Aided the Evolution of C4 Photosynthesis in Cleomaceae.Frontiers in plant science · 2020Article
- Posttranslational Modification of the NADP-Malic Enzyme Involved in CThe Plant cell · 2019Article
- Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells.Scientific reports · 2018Article
- Comparative cell-specific transcriptomics reveals differentiation of C4 photosynthesis pathways in switchgrass and other C4 lineages.Journal of experimental botany · 2016Article
- 2-Hydroxy Acids in Plant Metabolism.The arabidopsis book · 2015Article
- Biochemical approaches to C4 photosynthesis evolution studies: the case of malic enzymes decarboxylases.Photosynthesis research · 2013Review
- Kinetics and functional diversity among the five members of the NADP-malic enzyme family from Zea mays, a C4 species.Photosynthesis research · 2013Article
- Characterization of the NADP-malic enzymes in the woody plant Populus trichocarpa.Molecular biology reports · 2013Article
- Article
- Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana.The Journal of biological chemistry · 2010Article
- Evolutionary insights on C4 photosynthetic subtypes in grasses from genomics and phylogenetics.Genome biology and evolution · 2009Article
- Does Bienertia cycloptera with the single-cell system of C(4) photosynthesis exhibit a seasonal pattern of delta (13)C values in nature similar to co-existing C (4) Chenopodiaceae having the dual-cell (Kranz) system?Photosynthesis research · 2009Article
- Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots.Plant molecular biology · 2008Article
- Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties.Plant molecular biology · 2008Article
- Alteration of organic acid metabolism in Arabidopsis overexpressing the maize C4 NADP-malic enzyme causes accelerated senescence during extended darkness.Plant physiology · 2007Article
- Characterization of the NADP malic enzyme gene family in the facultative, single-cell C4 monocot Hydrilla verticillata.Photosynthesis research · 2007Article
- Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among the different isoforms of NADP-malic enzyme (NADP-ME) involved in a wide range of metabolic pathways in plants, the NADP-ME that participates in C(4)-photosynthesis is the most studied. In the present work, the expression in E. coli of a cDNA encoding for a maize non-photosynthetic NADP-ME is presented. The recombinant NADP-ME thus obtained presents kinetic and structural properties different from the enzyme previously purified from etiolated leaves and roots. Moreover, the recombinant non-photosynthetic NADP-ME presents very high intrinsic NADP-ME activity, which is unexpected for a non-C( 4) NADP-ME. Using antibodies against this recombinant enzyme, an immunoreactive band of 66 kDa is detected in different maize tissues indicating that the 66 kDa-NADP-ME is in fact a protein expressed in vivo. The recombinant NADP-ME assembles as a dimer, although the results obtained indicate that a higher molecular mass oligomeric state of the enzyme is found in maize roots in vivo. In this way, maize presents at least three NADP-ME isoforms: a 72 kDa constitutive form (previously characterized); the novel non-photosynthetic 66 kDa isoform characterized in this work (which is the product of the ZmChlMe2 gene and the likely precursor to the evolution of the photosynthetic C(4) NADP-ME) and the 62 kDa isoform (implicated in C(4) photosynthesis). The contribution of the present work anticipates further studies concerning the equilibrium between the oligomeric states of the NADP-ME isoforms and the evolution towards the C(4) isoenzyme in maize.
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