ArticleProceedings of the National Academy of Sciences of the United States of America2004
14-3-3 gamma associates with muscle specific kinase and regulates synaptic gene transcription at vertebrate neuromuscular synapse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Muscle-Specific Kinase Signaling and Its Therapeutic Potential.Muscle & nerve · 2026Review
- Pilot proteomic analysis of cerebrospinal fluid in Alzheimer's disease.Proteomics. Clinical applications · 2021Article
- Perversely expressed long noncoding RNAs can alter host response and viral proliferation in SARS-CoV-2 infection.Future virology · 2020Article
- Protein kinase CK2 interacts at the neuromuscular synapse with Rapsyn, Rac1, 14-3-3γ, and Dok-7 proteins and phosphorylates the latter two.The Journal of biological chemistry · 2015Article
- Global analysis of muscle-specific kinase signaling by quantitative phosphoproteomics.Molecular & cellular proteomics : MCP · 2014Article
- Selenoprotein W expression and regulation in mouse brain and neurons.Brain and behavior · 2013Article
- Disruption of basal lamina components in neuromotor synapses of children with spastic quadriplegic cerebral palsy.PloS one · 2013Article
- Molecular control of neuromuscular junction development.Journal of cachexia, sarcopenia and muscle · 2012Article
- The peripheral binding of 14-3-3γ to membranes involves isoform-specific histidine residues.PloS one · 2012Article
- Integrated genomics and proteomics of the Torpedo californica electric organ: concordance with the mammalian neuromuscular junction.Skeletal muscle · 2011Article
- Patient autoantibodies deplete postsynaptic muscle-specific kinase leading to disassembly of the ACh receptor scaffold and myasthenia gravis in mice.The Journal of physiology · 2010Article
- To build a synapse: signaling pathways in neuromuscular junction assembly.Development (Cambridge, England) · 2010Review
- ColQ controls postsynaptic differentiation at the neuromuscular junction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase, and negatively charged membranes.The Journal of biological chemistry · 2009Article
- Regulation of acetylcholine receptor clustering by ADF/cofilin-directed vesicular trafficking.Nature neuroscience · 2009Article
- Analysis of a Shc family adaptor protein, ShcD/Shc4, that associates with muscle-specific kinase.Molecular and cellular biology · 2007Article
- Regulation of synaptic growth and maturation by a synapse-associated E3 ubiquitin ligase at the neuromuscular junction.The Journal of cell biology · 2007Article
- Casein kinase 2-dependent serine phosphorylation of MuSK regulates acetylcholine receptor aggregation at the neuromuscular junction.Genes & development · 2006Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The muscle-specific receptor tyrosine kinase (MuSK) is part of a receptor complex, activated by neural agrin, that orchestrates the differentiation of the neuromuscular junction (NMJ). To gain insight into the function of the MuSK complex, we have developed a proteomic approach to identify new MuSK partners. MS analysis of MuSK crosslink products from postsynaptic membranes of the Torpedo electrocytes identified the adaptor protein 14-3-3 gamma. The 14-3-3 gamma protein was found localized at the adult rat NMJ. Cotransfection experiments in COS-7 cells showed that MuSK codistributed with the 14-3-3 gamma protein at the plasma membrane. Furthermore, 14-3-3 gamma was copurified by affinity chromatography with MuSK from transfected COS-7 cells and myotubes. The 14-3-3 gamma protein did not colocalize with agrin-elicited acetylcholine receptor (AChR) aggregates in cultured myotubes, suggesting that it is not involved in AChR clustering. Expression of 14-3-3 gamma specifically repressed the transcription of several synaptic reporter genes in cultured myotubes. This repression was potentiated by MuSK expression. Moreover, the expression of 14-3-3 gamma in muscle fibers in vivo caused both the repression of synaptic genes transcription and morphological perturbations of the NMJ. Our data extend the notion that, apart from its well documented role in AChR clustering, the MuSK complex might also be involved in the regulation of synaptic gene expression at the NMJ.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.