ArticleNucleic acids research2004
Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Therapeutic Oligonucleotides: An Outlook on Chemical Strategies to Improve Endosomal Trafficking.Cells · 2023Review
- Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells.Nucleic acids research · 2016Article
- miR-155 Overexpression Promotes Genomic Instability by Reducing High-fidelity Polymerase Delta Expression and Activating Error-Prone DSB Repair.Molecular cancer research : MCR · 2016Article
- Synthesis and triplex-forming properties of oligonucleotides capable of recognizing corresponding DNA duplexes containing four base pairs.Nucleic acids research · 2015Article
- Therapeutic genome mutagenesis using synthetic donor DNA and triplex-forming molecules.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Ultrasmall gold nanoparticles as carriers for nucleus-based gene therapy due to size-dependent nuclear entry.ACS nano · 2014Article
- Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases.Artificial DNA, PNA & XNA · 2014Article
- Targeted genome modification via triple helix formation.Methods in molecular biology (Clifton, N.J.) · 2014Article
- XPD-dependent activation of apoptosis in response to triplex-induced DNA damage.Nucleic acids research · 2013Article
- Local delivery of gene-modifying triplex-forming molecules to the epidermis.The Journal of investigative dermatology · 2013Article
- Targeted gene modification of hematopoietic progenitor cells in mice following systemic administration of a PNA-peptide conjugate.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Targeted disruption of the CCR5 gene in human hematopoietic stem cells stimulated by peptide nucleic acids.Chemistry & biology · 2011Article
- The triple helix: 50 years later, the outcome.Nucleic acids research · 2008Review
- Review
- Recent developments in peptide-based nucleic acid delivery.International journal of molecular sciences · 2008Article
- Sequence-specific triple helix formation with genomic DNA.Biochemistry · 2007Article
- Diels-Alder cycloadditions in water for the straightforward preparation of peptide-oligonucleotide conjugates.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Triplex-forming oligonucleotides (TFOs) are DNA-binding molecules, which offer the potential to selectively modulate gene expression. However, the biological activity of TFOs as potential antigene compounds has been limited by cellular uptake. Here, we investigate the effect of cell-penetrating peptides on the biological activity of TFOs as measured in an assay for gene-targeted mutagenesis. Using the transport peptide derived from the third helix of the homeodomain of antennapedia (Antp), we tested TFO-peptide conjugates compared with unmodified TFOs. TFOs covalently linked to Antp resulted in a 20-fold increase in mutation frequency when compared with 'naked' oligonucleotides. There was no increase above background in mutation frequency when Antp by itself was added to the cells or when Antp was linked to mixed or scrambled sequence control oligonucleotides. In addition, the TFO-peptide conjugates increased the mutation frequency of the target gene, and not the control gene, in a dose-responsive manner. Confocal microscopy using labeled oligonucleotides indicated increased cellular uptake of TFOs when linked to Antp, consistent with the gene-targeting data. These results suggest that peptide conjugation may enhance intranuclear delivery of reagents designed to bind to chromosomal DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.