ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Role of actin filaments in the axonal transport of microtubules.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
- An alternative splice isoform of mouse CDK5RAP2 induced cytoplasmic microtubule nucleation.IBRO neuroscience reports · 2022Article
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- Neuronal death in pneumococcal meningitis is triggered by pneumolysin and RrgA interactions with β-actin.PLoS pathogens · 2021Article
- Polarity Sorting of Microtubules in the Axon.Trends in neurosciences · 2018Review
- Intracellular trafficking pathways of Cx43 gap junction channels.Biochimica et biophysica acta. Biomembranes · 2018Review
- Polarity sorting of axonal microtubules: a computational study.Molecular biology of the cell · 2017Article
- Article
- MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility.Molecular biology of the cell · 2017Article
- Microtubules and Growth Cones: Motors Drive the Turn.Trends in neurosciences · 2016Review
- Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype.The Journal of cell biology · 2016Article
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- Transport and diffusion of Tau protein in neurons.Cellular and molecular life sciences : CMLS · 2014Review
- A micropatterning approach for imaging dynamic Cx43 trafficking to cell-cell borders.FEBS letters · 2014Article
- Classification of axonal subtypes based on cytoskeletal components.Cell health and cytoskeleton · 2014Article
- Modeling anterograde and retrograde transport of short mobile microtubules from the site of axonal branch formation.Journal of biological physics · 2014Article
- Growth cone-specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth.Neural development · 2013Article
- Mitotic motors coregulate microtubule patterns in axons and dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Unconventional functions of microtubule motors.Archives of biochemistry and biophysics · 2012Review
- Hooks and comets: The story of microtubule polarity orientation in the neuron.Developmental neurobiology · 2011Review
- Distortion of axonal cytoskeleton: an early sign of glaucomatous damage.Investigative ophthalmology & visual science · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules originate at the centrosome of the neuron and are then released for transport down the axon, in which they can move both anterogradely and retrogradely during axonal growth. It has been hypothesized that these movements occur by force generation against the actin cytoskeleton. To test this, we analyzed the movement, distribution, and orientation of microtubules in neurons pharmacologically depleted of actin filaments. Actin depletion reduced but did not eliminate the anterograde movements and had no effect on the frequency of retrograde movements. Consistent with the idea that microtubules might also move against neighboring microtubules, actin depletion completely inhibited the outward transport of microtubules under experimental conditions of low microtubule density. Interestingly, visualization of microtubule assembly shows that actin depletion actually enhances the tendency of microtubules to align with one another. Such microtubule-microtubule interactions are sufficient to orient microtubules in their characteristic polarity pattern in axons grown overnight in the absence of actin filaments. In fact, microtubule behaviors were only chaotic after actin depletion in peripheral regions of the neuron in which microtubules are normally sparse and hence lack neighboring microtubules with which they could interact. On the basis of these results, we conclude that microtubules are transported against either actin filaments or neighboring microtubules in the anterograde direction but only against other microtubules in the retrograde direction. Moreover, the transport of microtubules against one another provides a surprisingly effective option for the deployment and orientation of microtubules in the absence of actin filaments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.