ArticleMolecular biology of the cell2005
Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Two endogenousProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- Spontaneous signal generation by an excitable system for cell migration.Frontiers in cell and developmental biology · 2024Review
- Forty-five years of cGMP research inMolecular biology of the cell · 2021Review
- Short- and long-term memory of moving amoeboid cells.PloS one · 2021Article
- Signaling mechanisms for chemotaxis.Development, growth & differentiation · 2011Review
- Cellular responses to extracellular guidance cues.The EMBO journal · 2010Review
- A new Rac/PAK/GC/cGMP signaling pathway.Molecular and cellular biochemistry · 2010Review
- Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- ATP and NO dually control migration of microglia to nerve lesions.Developmental neurobiology · 2009Article
- Oscillatory signaling and network responses during the development of Dictyostelium discoideum.Ageing research reviews · 2008Review
- Big roles for small GTPases in the control of directed cell movement.The Biochemical journal · 2007Review
- Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells.Molecular biology of the cell · 2006Article
- Reversal of neuronal migration in a mouse model of fetal alcohol syndrome by controlling second-messenger signalings.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue of mammalian soluble adenylyl cyclase. sGC provides nearly all chemoattractant-stimulated cGMP formation and is essential for efficient chemotaxis toward cAMP. We show that in resting cells the major fraction of the sGC-GFP fusion protein localizes to the cytosol, and a small fraction is associated to the cell cortex. With the artificial substrate Mn2+/GTP, sGC activity and protein exhibit a similar distribution between soluble and particulate fraction of cell lysates. However, with the physiological substrate Mg2+/GTP, sGC in the cytosol is nearly inactive, whereas the particulate enzyme shows high enzyme activity. Reconstitution experiments reveal that inactive cytosolic sGC acquires catalytic activity with Mg2+/GTP upon association to the membrane. Stimulation of cells with cAMP results in a twofold increase of membrane-localized sGC-GFP, which is accompanied by an increase of the membrane-associated guanylyl cyclase activity. In a cAMP gradient, sGC-GFP localizes to the anterior cell cortex, suggesting that in chemotacting cells, sGC is activated at the leading edge of the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.