ArticleMolecular and cellular biology2005
Glucose inhibits meiotic DNA replication through SCFGrr1p-dependent destruction of Ime2p kinase.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Vir1p, the yeast homolog of virilizer, is required for mRNA m6A methylation and meiosis.Genetics · 2023Article
- Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Sporulation inJournal of fungi (Basel, Switzerland) · 2020Review
- Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.Microbial cell (Graz, Austria) · 2016Article
- Two-way communication between the metabolic and cell cycle machineries: the molecular basis.Cell cycle (Georgetown, Tex.) · 2015Review
- Nutritional control of growth and development in yeast.Genetics · 2012Review
- Coordination of cell growth and division by the ubiquitin-proteasome system.Seminars in cell & developmental biology · 2012Review
- The Sum1/Ndt80 transcriptional switch and commitment to meiosis in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2012Review
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
- The F-Box protein Fbp1 regulates sexual reproduction and virulence in Cryptococcus neoformans.Eukaryotic cell · 2011Article
- Ime1 and Ime2 are required for pseudohyphal growth of Saccharomyces cerevisiae on nonfermentable carbon sources.Molecular and cellular biology · 2010Article
- Ubiquitylation and proteasomal degradation of the p21(Cip1), p27(Kip1) and p57(Kip2) CDK inhibitors.Cell cycle (Georgetown, Tex.) · 2010Review
- Lessons from fungal F-box proteins.Eukaryotic cell · 2009Review
- The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae.Genetics · 2009Article
- Glucose induction pathway regulates meiosis in Saccharomyces cerevisiae in part by controlling turnover of Ime2p meiotic kinase.FEMS yeast research · 2008Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Evolution of Ime2 phosphorylation sites on Cdk1 substrates provides a mechanism to limit the effects of the phosphatase Cdc14 in meiosis.Molecular cell · 2007Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In the budding yeast Saccharomyces cerevisiae, the cell division cycle and sporulation are mutually exclusive cell fates; glucose, which stimulates the cell division cycle, is a potent inhibitor of sporulation. Addition of moderate concentrations of glucose (0.5%) to sporulation medium did not inhibit transcription of two key activators of sporulation, IME1 and IME2, but did increase levels of Sic1p, a cyclin-dependent kinase inhibitor, resulting in a block to meiotic DNA replication. The effects of glucose on Sic1p levels and DNA replication required Grr1p, a component of the SCF(Grr1p) ubiquitin ligase. Sic1p is negatively regulated by Ime2p kinase, and several observations indicate that glucose inhibits meiotic DNA replication through SCF(Grr1p)-mediated destruction of this kinase. First, Ime2p was destabilized in the presence of glucose, and this turnover required Grr1p, a second component of SCF(Grr1p), Cdc53p, and an SCF(Grr1p)-associated E2 enzyme, Cdc34p. Second, Ime2p-ubiquitin conjugates were detected under conditions of rapid Ime2p turnover, and conjugation of Ime2p to ubiquitin required GRR1. Third, a mutant form of Ime2p (Ime2(DeltaPEST)), in which a putative Grr1p-interacting sequence was deleted, was more stable than wild-type Ime2p. Finally, expression of the IME2(DeltaPEST) allele bypassed the block to meiotic DNA replication caused by 0.5% glucose. In addition, Grr1p is required for later events in sporulation independently of its role in Ime2p turnover.
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Registered trials
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